Recombinant Rhodopsin anticorps

N° du produit ABIN7072289

L’anticorps Souris Chimeric anti-Rhodopsin a été validé pour WB, IHC, ELISA, IP, IF et Lbl. Il convient pour détecter Rhodopsin dans des échantillons de Humain, Boeuf (Vache), Rat, Souris, Poisson zèbre (Danio rerio) et Amphibien.

Aperçu rapide pour Recombinant Rhodopsin anticorps (ABIN7072289)

Antigène: Rhodopsin (RHO)

Type d'anticorp: Recombinant Antibody

Reactivité: Humain, Boeuf (Vache), Rat, Souris, Poisson zèbre (Danio rerio), Amphibien

Hôte: Souris

Clonalité: Chimeric

Conjugué: Cet anticorp Rhodopsin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Labeling (Lbl)

Clone: Rho 1D4

Détail du produit anti-Rhodopsin anticorps

Fonction: Anti-Rhodopsin [Rho 1D4], Mouse IgG1, kappa

Specificité: The antibody binds to the C-terminus of rhodopsin on the cytoplasmic side of disk membranes. The epitope has the following amino acid sequence: 'TETSQVAPA'. A limitation of the 1D4 tag, however, is that it has to be placed at the C-terminus of a protein. This is because the Rho1D4 monoclonal antibody requires a free carboxylate group for high affinity binding. Amidation of the carboxyl group lowers its immunoreactivity to the Rho1D4 antibody by over 100 fold.

Réactivité croisée: Amphibien, Boeuf (Vache), Humain, Rat, Poisson zèbre (Danio rerio)

Attributs du produit:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogène: Bleached bovine rod outer segment (ROS).

Isotype: IgG1 kappa

Information d'application

Indications d'application: The antibody binds to the C-terminus of rhodopsin on the cytoplasmic side of disk membranes. Rhodopsin is the major pigment in rod photoreceptors and belongs to the family of G-protein-coupled receptors (GPCRs). The linear epitope (TETSQVAPA) can be used as a protein tag.

Commentaires:

This is a chimeric antibody created for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détails sur Rhodopsin

Antigène: Rhodopsin (RHO)

Autre désignation: Rhodopsin

Classe de substances: Chemical

Sujet: Rho-tag, Visual Purple

UniProt: P08100

Pathways: Signalisation WNT, Sensory Perception of Sound, Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction