Recombinant CD44 anticorps

N° du produit ABIN7072357

Cet anticorps Lapin Chimeric détecte spécifiquement CD44 dans FACS, WB, IHC, ELISA, IF, IP, ICC, Neut et BP. Il présente une réactivité envers Humain, Souris, Rat, Lapin, Boeuf (Vache), Mouton, Chévre et Primate.

Aperçu rapide pour Recombinant CD44 anticorps (ABIN7072357)

Antigène: CD44

Type d'anticorp: Recombinant Antibody

Reactivité: Humain, Souris, Rat, Lapin, Boeuf (Vache), Mouton, Chévre, Primate

Hôte: Lapin

Clonalité: Chimeric

Conjugué: Cet anticorp CD44 est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Neutralization (Neut), Blocking Peptide (BP)

Clone: Hermes-1

Détail du produit anti-CD44 anticorps

Fonction: Anti-CD44 [Hermes-1], Rabbit IgG, kappa

Specificité: This antibody is specific for CD44 (gp90 epitope cluster I), which is selectively expressed on normal or transformed lymphoid cells that are able to recognize and bind to high endothelial venules (specialized vessels that mediate lymphocyte exit from the blood into lymphoid organs). Although this antibody was raised against human CD44, it cross-reacts with murine, rat, rabbit, primate, sheep, bovine and goat CD44.

Réactivité croisée: Boeuf (Vache), Chévre, Souris, Primate, Lapin, Rat, Mouton

Attributs du produit:

Original Species of Ab: Rat

Original Format of Ab: IgG2a

Purification: Protein A affinity purified

Immunogène: This antibody was produced by immunizing a Fischer rat with human tonsil lymphocytes (10^7cells intraperitoneally in Freund's complete adjuvant). A boost was given 2 weeks later (intravascularly in saline).

Isotype: IgG kappa

Information d'application

Indications d'application: This antibody binds CD44, so can be used to label CD44+ cells in flow cytometry analysis. As CD44 Molecules are used to bind to high endothelial venules (HEV) at the site of lymphoctye entry to lymphoid organs, this antibody can be used to specifically stain HEV-binding cell lines (Jalkanen, 1986). In addition, this antibody blocks the adhesion of the haematopoietic progenitor cell line KG1 to hyaluronic acid (Bendall, 2003), but does not block lymphocyte binding to HEV (Picker, 1989). 7H9D6 partially blocks the binding of this antibody.

Commentaires:

This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détails sur CD44

Antigène: CD44

Autre désignation: CD44

Sujet: CDw44, Epican, Extracellular matrix receptor III, ECMR-III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, Phagocytic glycoprotein 1, PGP-1, Phagocytic glycoprotein I, CD44/H-CAM antibody, Hermes 1

UniProt: P16070

Pathways: Glycosaminoglycan Metabolic Process, Autophagy, Negative Regulation of intrinsic apoptotic Signaling