ISCU anticorps

N° du produit ABIN7115516

L’anticorps Lapin Polyclonal anti-ISCU a été validé pour WB, ELISA, IHC, IF et IP. Il convient pour détecter ISCU dans des échantillons de Humain, Souris et Rat.

Aperçu rapide pour ISCU anticorps (ABIN7115516)

Antigène: ISCU (Iron-sulfur cluster assembly enzyme ISCU, mitochondrial (ISCU))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ISCU est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Détail du produit anti-ISCU anticorps

Fonction: ISCU antibody

Purification: Immunogen affinity purified

Pureté: ≥95 % as determined by SDS-PAGE

Immunogène: iron-sulfur cluster scaffold homolog(E. coli)

Isotype: IgG

Information d'application

Indications d'application: WB: 1:500-1:2000, IP: 1:200-1:1000, IHC: 1:20-1:200, IF: 1:20-1:200

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze / thaw cycles.

Stock: -20 °C

Stockage commentaire: -20°C for 12 months

Date de péremption: 12 months

Détails sur ISCU

Antigène: ISCU (Iron-sulfur cluster assembly enzyme ISCU, mitochondrial (ISCU))

Autre désignation: ISCU

Sujet:

Synonyms: Iron-sulfur cluster assembly enzyme ISCU|NifU-like N-terminal domain-containing protein|NifU-like protein|ISCU|NIFUN

Background: Scaffold protein for the de novo synthesis of iron-sulfur(Fe-S) clusters within mitochondria, which is required for maturation of both mitochondrial and cytoplasmic [2Fe-2S] and [4Fe-4S] proteins(PubMed:11060020). First, a [2Fe-2S] cluster is transiently assembled on the scaffold protein ISCU. In a second step, the cluster is released from ISCU, transferred to a glutaredoxin GLRX5, followed by the formation of mitochondrial [2Fe-2S] proteins, the synthesis of [4Fe-4S] clusters and their target-specific insertion into the recipient apoproteins. Cluster assembly on ISCU depends on the function of the cysteine desulfurase complex NFS1-LYRM4/ISD11, which serves as the sulfur donor for cluster synthesis, the iron-binding protein frataxin as the putative iron donor, and the electron transfer chain comprised of ferredoxin reductase and ferredoxin, which receive their electrons from NADH(By similarity).

Poids moléculaire: 14-15 kDa, 18-19 kDa

ID gène: 23479

UniProt: Q9H1K1