PMS2 anticorps

N° du produit ABIN7117603

Cet anticorps Souris Monoclonal détecte spécifiquement PMS2 dans WB, ELISA et IHC. Il présente une réactivité envers Humain, Souris et Rat.

Aperçu rapide pour PMS2 anticorps (ABIN7117603)

Antigène: PMS2 (PMS2 Postmeiotic Segregation Increased 2 (S. Cerevisiae) (PMS2))

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PMS2 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Clone: 4B8

Détail du produit anti-PMS2 anticorps

Fonction: PMS2 antibody

Purification: Protein A+G purification

Pureté: ≥95 % as determined by SDS-PAGE

Immunogène: PMS2 postmeiotic segregation increased 2

Isotype: IgG1

Information d'application

Indications d'application: WB: 1:500-1:2000, IHC: 1:50-1:500

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze / thaw cycles.

Stock: -20 °C

Stockage commentaire: -20°C for 12 months

Date de péremption: 12 months

Détails sur PMS2

Antigène: PMS2 (PMS2 Postmeiotic Segregation Increased 2 (S. Cerevisiae) (PMS2))

Autre désignation: PMS2

Sujet:

Synonyms: Mismatch repair endonuclease PMS2|DNA mismatch repair protein PMS2|PMS1 protein homolog 2|PMS2|PMSL2

Background: Component of the post-replicative DNA mismatch repair system(MMR). Heterodimerizes with MLH1 to form MutL alpha. DNA repair is initiated by MutS alpha(MSH2-MSH6) or MutS beta(MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha(MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages.

Poids moléculaire: 100 kDa

ID gène: 5395

UniProt: P54278

Pathways: Réparation de l'ADN, Production of Molecular Mediator of Immune Response