TDG anticorps

N° du produit ABIN7119947

L’anticorps Lapin Polyclonal anti-TDG a été validé pour WB, ELISA et IP. Il convient pour détecter TDG dans des échantillons de Humain, Souris et Rat.

Aperçu rapide pour TDG anticorps (ABIN7119947)

Antigène: TDG (Thymine-DNA Glycosylase (TDG))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TDG est non-conjugé

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Détail du produit anti-TDG anticorps

Fonction: TDG antibody

Purification: Immunogen affinity purified

Pureté: ≥95 % as determined by SDS-PAGE

Immunogène: thymine-DNA glycosylase

Isotype: IgG

Information d'application

Indications d'application: WB: 1:500-1:2000, IP: 1:200-1:2000

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze / thaw cycles.

Stock: -20 °C

Stockage commentaire: -20°C for 12 months

Date de péremption: 12 months

Détails sur TDG

Antigène: TDG (Thymine-DNA Glycosylase (TDG))

Autre désignation: TDG

Sujet:

Synonyms: G/T mismatch-specific thymine DNA glycosylase|Thymine-DNA glycosylase (hTDG)|TDG

Background: DNA glycosylase that plays a key role in active DNA demethylation: specifically recognizes and binds 5-formylcytosine(5fC) and 5-carboxylcytosine(5caC) in the context of CpG sites and mediates their excision through base-excision repair(BER) to install an unmethylated cytosine. Cannot remove 5-hydroxymethylcytosine(5hmC). According to an alternative model, involved in DNA demethylation by mediating DNA glycolase activity toward 5-hydroxymethyluracil(5hmU) produced by deamination of 5hmC. Also involved in DNA repair by acting as a thymine-DNA glycosylase that mediates correction of G/T mispairs to G/C pairs: in the DNA of higher eukaryotes, hydrolytic deamination of 5-methylcytosine to thymine leads to the formation of G/T mismatches. Its role in the repair of canonical base damage is however minor compared to its role in DNA demethylation. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and a mispaired thymine. In addition to the G/T, it can remove thymine also from C/T and T/T mispairs in the order G/T >> C/T > T/T. It has no detectable activity on apyrimidinic sites and does not catalyze the removal of thymine from A/T pairs or from single-stranded DNA. It can also remove uracil and 5-bromouracil from mispairs with guanine.

Poids moléculaire: 55-60 kDa

ID gène: 6996

UniProt: Q13569

Pathways: Réparation de l'ADN, Chromatin Binding