TRIM5 anticorps

N° du produit ABIN7120293

Cet anticorps anti-TRIM5 est un anticorps Lapin Polyclonal détectant TRIM5 dans WB, ELISA et IHC. Adapté pour Humain et Souris.

Aperçu rapide pour TRIM5 anticorps (ABIN7120293)

Antigène: TRIM5 (Tripartite Motif Containing 5 (TRIM5))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TRIM5 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Détail du produit anti-TRIM5 anticorps

Fonction: TRIM5 antibody

Purification: Immunogen affinity purified

Pureté: ≥95 % as determined by SDS-PAGE

Immunogène: tripartite motif-containing 5

Isotype: IgG

Information d'application

Indications d'application: WBL:1:200-1:1000, IHC: 1:20-1:200

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze / thaw cycles.

Stock: -20 °C

Stockage commentaire: -20°C for 12 months

Date de péremption: 12 months

Détails sur TRIM5

Antigène: TRIM5 (Tripartite Motif Containing 5 (TRIM5))

Autre désignation: TRIM5

Sujet:

Synonyms: Tripartite motif-containing protein 5|RING finger protein 88|RING-type E3 ubiquitin transferase TRIM5|TRIM5|RNF88

Background: Capsid-specific restriction factor that prevents infection from non-host-adapted retroviruses. Blocks viral replication early in the life cycle, after viral entry but before reverse transcription. In addition to acting as a capsid-specific restriction factor, also acts as a pattern recognition receptor that activates innate immune signaling in response to the retroviral capsid lattice. Binding to the viral capsid triggers its E3 ubiquitin ligase activity, and in concert with the heterodimeric ubiquitin conjugating enzyme complex UBE2V1-UBE2N(also known as UBC13-UEV1A complex) generates 'Lys-63'-linked polyubiquitin chains, which in turn are catalysts in the autophosphorylation of the MAP3K7/TAK1 complex(includes TAK1, TAB2, and TAB3). Activation of the MAP3K7/TAK1 complex by autophosphorylation results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes, thereby leading to an innate immune response in the infected cell. Restricts infection by N-tropic murine leukemia virus(N-MLV), equine infectious anemia virus(EIAV), simian immunodeficiency virus of macaques(SIVmac), feline immunodeficiency virus(FIV), and bovine immunodeficiency virus(BIV)(PubMed:17156811). Plays a role in regulating autophagy through activation of autophagy regulator BECN1 by causing its dissociation from its inhibitors BCL2 and TAB2(PubMed:25127057). Also plays a role in autophagy by acting as a selective autophagy receptor which recognizes and targets HIV-1 capsid protein p24 for autophagic destruction(PubMed:25127057).

Poids moléculaire: 70 kDa, 46 kDa

ID gène: 85363

UniProt: Q9C035

Pathways: Activation of Innate immune Response