Clusterin anticorps (AA 150-300)

N° du produit ABIN7126231

Détail du produit anti-Clusterin anticorps

Antigène: Clusterin (CLU)

Épitope: AA 150-300

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Clusterin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificité: Clusterin, also designated complement lysis inhibitor (CLI), apolipoprotein J (APOJ), sulfated glycoprotein 2 (SGP2), SP40 and testosterone-repressed prostate message 2 (TRPM2), is a secretory, heterodimeric glycoprotein that influences immune regulation, cell adhesion, transformation, lipid transportation, tissue remodeling, membrane recycling and cell-cell interactions. Clusterin is synthesized as a 449 amino acid polypeptide that is post-translationally cleaved at an internal bond between Arg 227 and Ser 228. Two subunits, i± and i², are associated through disulfide bonds. The i² subunit (also called ApoJi±) corresponds to residues 23-227. The i± subunit (also called ApoJi²) corresponds to residues 228-449. Overexpression of Clusterin appears to be more common in late stages of mammary tumor progression. Clusterin markedly influences i²-Amyloid structure and neuritic toxicity in vivo and may influence Alzheimer s pathogenesis.

Réactivité croisée (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant fragment (around aa150-300) of human CLU protein (exact sequence is proprietary)

Isotype: IgG2b

Information d'application

Indications d'application:

Known_Application: Western Blot (2-4 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human pancreas, tonsil or cervix.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur Clusterin

Antigène: Clusterin (CLU)

Autre désignation: CLU (CLU Produits)

Synonymes: anticorps LOC100136120, anticorps clu, anticorps MGC53055, anticorps zgc:73352, anticorps xclu, anticorps MGC89163, anticorps CLU, anticorps APO-J, anticorps APOJ, anticorps CLI, anticorps CLU1, anticorps CLU2, anticorps KUB1, anticorps NA1/NA2, anticorps SGP-2, anticorps SGP2, anticorps SP-40, anticorps TRPM-2, anticorps TRPM2, anticorps DAG, anticorps RATTRPM2B, anticorps SP40, anticorps TRPM2B, anticorps Trpm2, anticorps Trpmb, anticorps AI893575, anticorps ApoJ, anticorps Cli, anticorps D14Ucla3, anticorps Sgp-2, anticorps Sgp2, anticorps Sugp-2, anticorps GP80, anticorps gpIII, anticorps 40, anticorps clusterin-1, anticorps clusterin L homeolog, anticorps clusterin, anticorps Clusterin, anticorps LOC100136120, anticorps clu.L, anticorps clu, anticorps CLU, anticorps clus, anticorps Clu, anticorps LOC101113728

Sujet: Aging associated protein 4, Apolipoprotein J, CLU1, CLU2, Clusterin alpha / beta chain, Complement associated protein SP40, Complement cytolysis inhibitor a chain, NA1/NA2, D14Ucla3, Dimeric acid glycoprotein, GP80, Ku70-binding protein 1 (KUB1), Sulfated glycoprotein 2 (SGP2), Testosterone repressed prostate message 2 (TPRM2B),Clusterin / Apolipoprotein J (APO-J)
Cellular localisation: Secreted. Can retrotranslocate from secretory compartments to cytosol upon cellular stress and Nucleus. Cytoplasm. Mitochondrion membrane. Cytoplasm, cytosol. Microsome. Endoplasmic reticulum. Cytopla

Poids moléculaire: 70kDa (precursor), 36-39kDa (i±), 34-36kDa (i²)

ID gène: 1191, 436657

UniProt: P10909

Pathways: Apoptose, Negative Regulation of intrinsic apoptotic Signaling