HDAC1 anticorps

N° du produit ABIN7126509

L’anticorps Souris Monoclonal anti-HDAC1 a été validé pour ELISA, IF, IP, FACS et Coat. Il convient pour détecter HDAC1 dans des échantillons de Humain.

Aperçu rapide pour HDAC1 anticorps (ABIN7126509)

Antigène: HDAC1 (Histone Deacetylase 1 (HDAC1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp HDAC1 est non-conjugé

Application: ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS), Coating (Coat)

Détail du produit anti-HDAC1 anticorps

Specificité: In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (for p300/ CBP-associated factor), p300/CBP and the TFIID subunit TAFII p250. Mammalian HDAC1 (also designated HD1) and HDAC2 (also designated mammalian RPD3), both of which are related to the yeast transcriptional regulator Rpd3p, have been identified as histone deacetylases.

Réactivité croisée (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant full-length human HDAC1 protein

Isotype: IgG

Information d'application

Indications d'application:

Known_Application: ELISA (For coating, order antibody without BSA), ,Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), ,Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or Jurkat cells.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur HDAC1

Antigène: HDAC1 (Histone Deacetylase 1 (HDAC1))

Autre désignation: HDAC1

Sujet: DKFZp686H12203, GON 10, HD1, Reduced potassium dependency yeast homolog like 1 (RPD3L1), RPD3,Histone Deacetylase 1 (HDAC1)
Cellular localisation: Nucleus. Nucleoplasm.

Poids moléculaire: 60kDa

ID gène: 3065, 88556

UniProt: Q13547

Pathways: Neurotrophin Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Mitotic G1-G1/S Phases, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Negative Regulation of intrinsic apoptotic Signaling, Embryonic Body Morphogenesis