Recombinant IDH1 anticorps (AA 281-414)

N° du produit ABIN7126537

Cet anticorps anti-IDH1 est un anticorps Lapin Monoclonal détectant IDH1 dans IHC (f). Adapté pour Humain.

Aperçu rapide pour Recombinant IDH1 anticorps (AA 281-414) (ABIN7126537)

Antigène: IDH1 (Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp IDH1 est non-conjugé

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Détail du produit anti-IDH1 anticorps

Épitope: AA 281-414

Specificité: IDH1 R132H antibody binds to IDH1-mutated protein, but does not bind the wild-type IDH1 protein. IDH1 R132H point mutations are frequently seen in World Health Organization grade II and III gliomas and are believed to constitute an early step in tumorigenesis. IDH1 R132H can be used as a diagnostic marker to help differentiate infiltrating gliomas from gliosis, and as a prognostic marker for gliomas and secondary glioblastoma multiforme. IDH1 R132H antibody shows strong cytoplasmic staining and weaker nuclear staining in tumor cells with the R132H-mutated peptide. Diffuse staining of the fibrillary tumor matrix is also seen.

Réactivité croisée (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant fragment of human IDH1 protein (around aa 281-414) (exact sequence is proprietary)

Isotype: IgG

Information d'application

Indications d'application:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HepG2, HeLa, HT29 or MCF7cells. Humanbreast, colon or prostate carcinoma.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur IDH1

Antigène: IDH1 (Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1))

Autre désignation: IDH1

Sujet: Cytosolic NADP-isocitrate dehydrogenase, Epididymis luminal protein 216, Epididymis secretory protein Li 26, HEL-216, HEL-S-26, ICDH, IDCD, IDH1, IDP, IDPC, Isocitrate dehydrogenase 1 (NADP+) soluble, NADP dependent isocitrate dehydrogenase peroxisomal, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase, PICD,IDH1-R132H (Isocitrate Dehydrogenase)
Cellular localisation: Cytoplasm. Nucleus.

Poids moléculaire: 45-47kDa

ID gène: 3417, 593422

UniProt: O75874

Pathways: L'effet Warburg