NME1 anticorps

N° du produit ABIN7126749

L’anticorps Souris Monoclonal anti-NME1 a été validé pour WB, ELISA, Coat et IHC (f). Il convient pour détecter NME1 dans des échantillons de Humain.

Aperçu rapide pour NME1 anticorps (ABIN7126749)

Antigène: NME1 (Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp NME1 est non-conjugé

Application: Western Blotting (WB), ELISA, Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Détail du produit anti-NME1 anticorps

Specificité: The nm23 gene, a potential suppressor of metastasis, was originally identified by differential hybridization between two murine melanoma sub-lines, one with a high and the second with a low metastatic capacity. Highly metastatic sub-lines exhibit much lower levels of nm23 than less metastatic cells. Based on sequence analysis, nm23 appears highly related to nucleotide diphosphate kinases (NDP). In humans, NDP kinases A and B are identical to two isotypes of human nm23 homologs, namely nm23-H1 and H2, respectively. nm23-H2 is identical in sequence to PuF, a transcription factor that binds to nuclease hypersensitive elements at positions 142 to 115 of the human c-Myc promotor.

Réactivité croisée (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant full-length human NME1 protein

Isotype: IgG2b

Information d'application

Indications d'application:

Known_Application: ELISA (For coating, order antibody without BSA), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HeLa, A549 or Jurkat cells. Ubiquitously expressed in all tissues.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur NME1

Antigène: NME1 (Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1))

Autre désignation: NME1

Sujet: AWD, Granzyme A activated DNase (GAAD), GZMA activated DNase, Metastasis inhibition factor NM23, NBS, NDKA, NM23, nm23-H1, NM23-M1, NM23H1B, included, NME/NM23 nucleoside diphosphate kinase 1, Nme1, NME1-NME2 spliced read-through transcript, included, Non-metastatic cells 1, protein (NM23A) expressed in, Nonmetastatic cells 1, protein expressed in, Nonmetastatic protein 23, Tumor metastatic process-associated protein,Nucleoside Diphosphate Kinase A / nm23-H1
Cellular localisation: Cytoplasm. Nucleus. Cell-cycle dependent nuclear localization.

Poids moléculaire: 17kDa

ID gène: 4830, 463456

UniProt: P15531

Pathways: Apoptose, Nucleotide Phosphorylation, Carbohydrate Homeostasis, Ribonucleoside Biosynthetic Process