PCNA anticorps

N° du produit ABIN7126768

Cet anticorps anti-PCNA Monoclonal Souris (Clone PC10) (ABIN7126768) détecte spécifiquement PCNA dans WB, IHC et FACS. L’anticorps est réactif avec des échantillons de Humain, Souris, Rat, Poulet, Porc, Singe, Saccharomyces cerevisiae, Lapin, Poisson zèbre (Danio rerio), Drosophila melanogaster, Schizosaccharomyces pombe, Hamster et Cobaye.

Aperçu rapide pour PCNA anticorps (ABIN7126768)

Antigène: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reactivité: Humain, Souris, Rat, Poulet, Porc, Singe, Saccharomyces cerevisiae, Lapin, Poisson zèbre (Danio rerio), Drosophila melanogaster, Schizosaccharomyces pombe, Hamster, Cobaye

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PCNA est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Clone: PC10

Détail du produit anti-PCNA anticorps

Fonction: PCNA (Proliferating Cell Nuclear Antigen) (G1- & S-phase Marker) Antibody

Specificité: Recognizes a non-histone protein of 36 kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This MAb is excellent for multiple applications.

Réactivité croisée (Details): Human. Monkey. Pig. Mouse. Rat. Chicken. Zebrafish. Drosophila melanogaster. Yeast (S. pombe. S. cerevisiae). Dog

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Rat PCNA/protein A fusion protein

Isotype: IgG2a, kappa

Information d'application

Indications d'application:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: A549, Tonsil or reactive lymph node.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur PCNA

Antigène: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Autre désignation: PCNA

Sujet:

Cyclin, DNA polymerase delta auxiliary protein, Mutagen-sensitive 209 protein, PCNAR, Polymerase delta accessory protein

A-431, 293T, Hep G2, MCF7, NIH/3T3, A549, Raji cells, Human Tonsil, Human Spleen or kidney

Poids moléculaire: 36kDa

ID gène: 5111, 147433, 728886

UniProt: P12004

Pathways: Telomere Maintenance, Réparation de l'ADN, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy