Recombinant PMS2 anticorps (AA 1-100)

N° du produit ABIN7126800

L’anticorps Lapin Monoclonal anti-PMS2 a été validé pour WB, IF, FACS et IHC (f). Il convient pour détecter PMS2 dans des échantillons de Humain.

Aperçu rapide pour Recombinant PMS2 anticorps (AA 1-100) (ABIN7126800)

Antigène: PMS2 (PMS2 Postmeiotic Segregation Increased 2 (S. Cerevisiae) (PMS2))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp PMS2 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Détail du produit anti-PMS2 anticorps

Épitope: AA 1-100

Specificité: PMS2 is involved in DNA mismatch repair. It forms a heterodimer with MLH1 and this complex interacts with other complexes bound to mismatched bases. Defects in PMS2 are the cause of hereditary non-polyposis colorectal cancer type 4 (HNPCC4). Mutations in more than one gene locus can be involved alone or in combination in the production of the HNPCC phenotype (also called Lynch syndrome). Most families with clinically recognized HNPCC have mutations in either MLH1 or MSH2 genes. HNPCC is an autosomal, dominantly inherited disease associated with marked increase in cancer susceptibility. It is characterized by a familial predisposition to early onset colorectal carcinoma (CRC) and extra-colonic cancers of the gastrointestinal, urological and female reproductive tracts. HNPCC is reported to be the most common form of inherited colorectal cancer in the Western world, and accounts for 15 % of all colon cancers. Defects in PMS2 are a cause of mismatch repair cancer syndrome (MMRCS), also known as Turcot syndrome or brain tumor-polyposis syndrome 1 (BTPS1). MMRCS is an autosomal dominant disorder characterized by malignant tumors of the brain associated with multiple colorectal adenomas. Skin features include sebaceous cysts, hyperpigmented and cafe au lait spots.

Réactivité croisée (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: A synthetic peptide corresponding to residues within aa1-100 of human PMS2 (exact sequence is proprietary)

Isotype: IgG

Information d'application

Indications d'application:

Known_Application: Flow Cytometry (1-2 μg/million cells), Western Blot (1-2 μg/mL), Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or Jurkat cells. Human colon carcinoma.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur PMS2

Antigène: PMS2 (PMS2 Postmeiotic Segregation Increased 2 (S. Cerevisiae) (PMS2))

Autre désignation: PMS2

Sujet: DNA mismatch repair protein PMS2, hereditary non-polyposis colorectal cancer type 4 (HNPCC4), Mismatch repair gene PMSL2, PMS1 protein homolog 2, postmeiotic segregation increased 2 (S. cerevisiae), PMS2CL,PMS2 (Postmeiotic Segregation Increased 2)
Cellular localisation: Nuclear

Poids moléculaire: 96kDa

ID gène: 5395, 632637

UniProt: P54278

Pathways: Réparation de l'ADN, Production of Molecular Mediator of Immune Response