PKC iota anticorps (AA 100-300)

N° du produit ABIN7126834

Cet anticorps anti-PKC iota est un anticorps Souris Monoclonal détectant PKC iota dans IHC (f). Adapté pour Humain.

Aperçu rapide pour PKC iota anticorps (AA 100-300) (ABIN7126834)

Antigène: PKC iota (PRKCI) (Protein Kinase C, iota (PRKCI))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PKC iota est non-conjugé

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Détail du produit anti-PKC iota anticorps

Épitope: AA 100-300

Specificité: Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions, including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes, including conventional (c) PKC isoforms (i±, i²I, i²II and i³) and novel (n) PKC isoforms ( L, , A•, , , l/i, m and n). Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of PKC L and are independent of Ca2+. On the other hand, most of the other PKC members possess phorbol ester-binding activities and kinase activities.

Réactivité croisée (Details): Human, Cat and Dog.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant fragment (around aa100-300) of human PRKCI protein (exact sequence is proprietary)

Isotype: IgG2b

Information d'application

Indications d'application:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Brain, liver or placenta.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur PKC iota

Antigène: PKC iota (PRKCI) (Protein Kinase C, iota (PRKCI))

Autre désignation: PRKCI

Sujet: APKC lambda/iota, Atypical protein kinase C lambda/iota, Atypical protein kinase C-lambda/iota, nPKC-iota, nPKCiota, PKC lambda/iota, PKCI, PKCiota, PKClambda, PRKC iota, PRKC lambda/iota, PRKCI, Protein kinase C iota type,Protein Kinase C iota / lambda / PRKCI
Cellular localisation: Cytoplasm. Cell Membrane. Nucleus.

Poids moléculaire: 68kDa

ID gène: 5584, 478199

UniProt: P41743

Pathways: Neurotrophin Signaling Pathway, Cell-Cell Junction Organization, Tube Formation