Recombinant Cyclin D1 anticorps (AA 270-295)

N° du produit ABIN7126874

Cet anticorps Lapin Monoclonal détecte spécifiquement Cyclin D1 dans WB et IHC. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour Recombinant Cyclin D1 anticorps (AA 270-295) (ABIN7126874)

Antigène: Cyclin D1 (CCND1)

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp Cyclin D1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: CCND1-3370R

Détail du produit anti-Cyclin D1 anticorps

Épitope: AA 270-295

Fonction: Recombinant Cyclin D1 (G1-Cyclin & Mantle Cell Lymphoma Marker) Antibody

Specificité: Recognizes a protein of 36 kDa, identified as cyclin D1. Cyclin D1, one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. This antibody neutralizes the activity of cyclin D1 in vivo. About 60 % of mantle cell lymphomas (MCL) contain a t(11, 14)(q13, q32) translocation resulting in over-expression of cyclin D1. This antibody is useful in identifying mantle cell lymphomas (cyclin D1 positive) from CLL/SLL and follicular lymphomas (cyclin D1 negative). Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1.

Réactivité croisée (Details): Human.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Synthetic peptide corresponding to residues within aa 270-295 of Cyclin D1

Isotype: IgG, kappa

Information d'application

Indications d'application:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human mantle cell lymphoma, breast or bladder carcinomas.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur Cyclin D1

Antigène: Cyclin D1 (CCND1)

Autre désignation: CCND1

Sujet:

B cell CLL/lymphoma 1, B cell leukemia 1, B-cell lymphoma 1 protein, BCL-1 oncogene, CCND1 protein, CCND1/FSTL3 fusion gene, CCND1/IGHG1 fusion gene CCND1/IGLC1 fusion gene, CCND1/PTH fusion gene, G1/S-specific cyclin-D1, Parathyroid adenomatosis 1, PRAD1 oncogene

RAW 264.7.,Breast or Bladder Carcinomas., Human mantle cell lymphoma, HeLa, A431.

Poids moléculaire: 36kDa

ID gène: 595

UniProt: P24385

Pathways: Signalisation PI3K-Akt, Cycle Cellulaire, Mitotic G1-G1/S Phases, ER-Nucleus Signaling