SOX4 anticorps (AA 45-130)

N° du produit ABIN7126957

L’anticorps Souris Monoclonal anti-SOX4 a été validé pour WB, IF, FACS, IP et IHC (f). Il convient pour détecter SOX4 dans des échantillons de Humain.

Aperçu rapide pour SOX4 anticorps (AA 45-130) (ABIN7126957)

Antigène: SOX4 (SRY (Sex Determining Region Y)-Box 4 (SOX4))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp SOX4 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Détail du produit anti-SOX4 anticorps

Épitope: AA 45-130

Specificité: SOX4 is a member of the SOX (SRY-related HMG-box) family of transcription factors with a critical role in embryonic development and in cell-fate determination during organogenesis of the heart, pancreas, brain, and in B and T lymphocyte differentiation. SOX4 gene expression is upregulated in many cancer types, and increased SOX4 activity contributes to cellular transformation, cell survival, and metastasis. Gene expression profiling has uncovered SOX4 with upregulated activity during TGF-i²-induced epithelial-mesenchymal transition (EMT) in normal and cancerous breast epithelial cells. SOX4 is indispensable for EMT and cell survival invitro and for primary tumor growth and metastasis invivo. SOX4 is identified as a master regulator of EMT by governing the expression of the epigenetic modifier Ezh2.

Réactivité croisée (Details): Human. Predicted to react in Xenopus and Chick.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant fragment (around aa45-130) of human SOX4 protein

Isotype: IgG2b

Information d'application

Indications d'application:

Known_Application: Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), ,Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HeLa cells. Colon or breast carcinoma.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur SOX4

Antigène: SOX4 (SRY (Sex Determining Region Y)-Box 4 (SOX4))

Autre désignation: SOX4

Sujet: SOX, SRY (sex determining region Y) box 4, SRY box containing gene 4, SRY related HMG box gene 4, Transcription factor SOX-4,SOX4 (Master Regulator of Epithelial-Mesenchymal Transition)
Cellular localisation: Nucleus.

Poids moléculaire: 47kDa

ID gène: 6659, 643910

UniProt: Q06945