Recombinant Topoisomerase II alpha anticorps (AA 1352-1493)
-
- Antigène Voir toutes Topoisomerase II alpha (TOP2A) Anticorps
- Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))
- Type d'anticorp
- Recombinant Antibody
-
Épitope
- AA 1352-1493
-
Reactivité
- Humain
-
Hôte
- Souris
-
Clonalité
- Monoclonal
-
Conjugué
- Cet anticorp Topoisomerase II alpha est non-conjugé
-
Application
- Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Specificité
- It recognizes a 170 kDa protein, which is identified as topoisomerase IIi±. It shows no cross-reaction with Topoisomerase II i² or I. Topo II i± plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo II i± is also implicated in drug resistance of tumor cells and has been shown to be over-expressed in many human cancers. Decreased expression of Topo IIa is the predominant mechanism of resistance to several chemotherapeutic agents.
- Réactivité croisée (Details)
- Human. Mouse.
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogène
- Recombinant human Topoisomerase II alpha fragment (aa1352-1493)
- Isotype
- IgG2a
- Top Product
- Discover our top product TOP2A Anticorps primaire
-
-
- Indications d'application
-
Known_Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: HeLa or Jurkat cells. Human lymph node or tonsil.
- Restrictions
- For Research Use only
-
- Concentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Agent conservateur
- Azide free
- Stock
- -20 °C,-80 °C
- Stockage commentaire
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Date de péremption
- 24 months
-
- Antigène
- Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))
- Autre désignation
- TOP2A (TOP2A Produits)
- Synonymes
- anticorps TOP2, anticorps TP2A, anticorps TOP2A, anticorps Top-2, anticorps TOP-2, anticorps F12B17.110, anticorps F12B17_110, anticorps thimet metalloendopeptidase 2, anticorps fc08c07, anticorps wu:fc08c07, anticorps DNA topoisomerase II alpha, anticorps DNA topoisomerase II, alpha isozyme, anticorps topoisomerase (DNA) II alpha, anticorps Zincin-like metalloproteases family protein, anticorps TOP2A, anticorps Bm1_08705, anticorps Top2a, anticorps AT5G10540, anticorps top2a
- Sujet
-
ATP hydrolyzing DNA topoisomerase II alfa, DNA gyrase, DNA topoisomerase (ATP hydrolyzing), DNA topoisomerase 2 alpha, DNA topoisomerase II 170kD, DNA topoisomerase II alpha, DNA Topoisomerase2, TOP2A, Topoisomerase DNA II alpha 170 kDa, TP2A,Topoisomerase II alpha (Proliferation & Drug-Resistance Marker)
Cellular localisation: Nuclear - Poids moléculaire
- 170kDa
- ID gène
- 7153, 156346
- UniProt
- P11388
- Pathways
- Cycle Cellulaire, Mitotic G1-G1/S Phases
-