Recombinant Topoisomerase II alpha anticorps (AA 1352-1493)

N° du produit ABIN7127010

Cet anticorps Souris Monoclonal détecte spécifiquement Topoisomerase II alpha dans IHC. Il présente une réactivité avec des échantillons de Humain et Souris.

Aperçu rapide pour Recombinant Topoisomerase II alpha anticorps (AA 1352-1493) (ABIN7127010)

Antigène: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Topoisomerase II alpha est non-conjugé

Application: Immunohistochemistry (IHC)

Clone: RTOP2A-6629

Détail du produit anti-Topoisomerase II alpha anticorps

Épitope: AA 1352-1493

Fonction: Recombinant Topoisomerase II alpha (Proliferation & Drug-Resistance Marker) Antibody

Specificité: It recognizes a 170kDa protein, which is identified as topoisomerase II°™. It shows no cross-reaction with Topoisomerase II æ or I. Topo II °™ plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo II °™ is also implicated in drug resistance of tumor cells and has been shown to be over-expressed in many human cancers. Decreased expression of Topo IIa is the predominant mechanism of resistance to several chemotherapeutic agents.

Réactivité croisée (Details): Human and Mouse.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Recombinant human Topoisomerase II alpha fragment (aa1352-1493)

Isotype: IgG1, kappa

Information d'application

Indications d'application:

Known_Application: Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human lymph node or tonsil.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur Topoisomerase II alpha

Antigène: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Autre désignation: TOP2A

Sujet:

ATP hydrolyzing DNA topoisomerase II alfa, DNA gyrase, DNA topoisomerase (ATP hydrolyzing), DNA topoisomerase 2 alpha, DNA topoisomerase II 170kD, DNA topoisomerase II alpha, DNA Topoisomerase2, TOP2A, Topoisomerase DNA II alpha 170 kDa, TP2A

Human lymph node or tonsil.

Poids moléculaire: 170kDa

ID gène: 7153, 156346

UniProt: P11388

Pathways: Cycle Cellulaire, Mitotic G1-G1/S Phases