Recombinant Topoisomerase II alpha anticorps (AA 1352-1493)

N° du produit ABIN7127012

Cet anticorps anti-Topoisomerase II alpha Monoclonal Lapin (Clone TOP2A-6570R) (ABIN7127012) détecte spécifiquement Topoisomerase II alpha dans WB et IHC. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour Recombinant Topoisomerase II alpha anticorps (AA 1352-1493) (ABIN7127012)

Antigène: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp Topoisomerase II alpha est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: TOP2A-6570R

Détail du produit anti-Topoisomerase II alpha anticorps

Épitope: AA 1352-1493

Fonction: Recombinant Topoisomerase II alpha (Proliferation & Drug-Resistance Marker) Antibody

Specificité: The topoisomerase II-°™ isoform is a 170kDa nuclear protein and plays an important role in DNA synthesis and RNA transcription, as well as chromosomal segregation during mitosis. Topoisomerase II-°™ is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells, and has been shown to be over-expressed in many human cancers. Decreased expression of Topoisomerase II-°™ is the predominant mechanism of resistance to several chemotherapeutic agents.

Réactivité croisée (Details): Human.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Recombinant human Topoisomerase II alpha fragment (aa1352-1493) (exact sequence is proprietary)

Isotype: IgG, kappa

Information d'application

Indications d'application:

Known_Application: Western Blot (2-4 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or Jurkat cells. Human tonsil or esophagus.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur Topoisomerase II alpha

Antigène: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Autre désignation: TOP2A

Sujet:

ATP hydrolyzing DNA topoisomerase II alfa, DNA gyrase, DNA topoisomerase (ATP hydrolyzing), DNA topoisomerase 2 alpha, DNA topoisomerase II 170kD, DNA topoisomerase II alpha, Topoisomerase DNA II alpha 170 kDa, TP2A

HeLa or Jurkat cells. Human tonsil or esophagus.

Poids moléculaire: 170kDa

ID gène: 7153, 156346

UniProt: P11388

Pathways: Cycle Cellulaire, Mitotic G1-G1/S Phases