Recombinant Topoisomerase II alpha anticorps (AA 1352-1493)

N° du produit ABIN7127012

Détail du produit anti-Topoisomerase II alpha anticorps

Antigène: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Type d'anticorp: Recombinant Antibody

Épitope: AA 1352-1493

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp Topoisomerase II alpha est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificité: The topoisomerase II-i± isoform is a 170 kDa nuclear protein and plays an important role in DNA synthesis and RNA transcription, as well as chromosomal segregation during mitosis. Topoisomerase II-i± is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells, and has been shown to be over-expressed in many human cancers. Decreased expression of Topoisomerase II-i± is the predominant mechanism of resistance to several chemotherapeutic agents.

Réactivité croisée (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant human Topoisomerase II alpha fragment (aa1352-1493) (exact sequence is proprietary)

Isotype: IgG

Information d'application

Indications d'application:

Known_Application: Western Blot (2-4 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or Jurkat cells. Human tonsil or esophagus.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur Topoisomerase II alpha

Antigène: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Autre désignation: TOP2A (TOP2A Produits)

Synonymes: anticorps TOP2, anticorps TP2A, anticorps TOP2A, anticorps Top-2, anticorps TOP-2, anticorps F12B17.110, anticorps F12B17_110, anticorps thimet metalloendopeptidase 2, anticorps fc08c07, anticorps wu:fc08c07, anticorps DNA topoisomerase II alpha, anticorps DNA topoisomerase II, alpha isozyme, anticorps topoisomerase (DNA) II alpha, anticorps Zincin-like metalloproteases family protein, anticorps TOP2A, anticorps Bm1_08705, anticorps Top2a, anticorps AT5G10540, anticorps top2a

Sujet: ATP hydrolyzing DNA topoisomerase II alfa, DNA gyrase, DNA topoisomerase (ATP hydrolyzing), DNA topoisomerase 2 alpha, DNA topoisomerase II 170kD, DNA topoisomerase II alpha, Topoisomerase DNA II alpha 170 kDa, TP2A,Topoisomerase II alpha (Proliferation & Drug-Resistance Marker)
Cellular localisation: Nuclear

Poids moléculaire: 170kDa

ID gène: 7153, 156346

UniProt: P11388

Pathways: Cycle Cellulaire, Mitotic G1-G1/S Phases