Recombinant Caldesmon anticorps

N° du produit ABIN7127077

L’anticorps anti-Caldesmon Monoclonal Souris est utilisé pour la détection de Caldesmon dans des échantillons de Humain. Il a été validé pour WB.

Aperçu rapide pour Recombinant Caldesmon anticorps (ABIN7127077)

Antigène: Caldesmon (CALD1) (Caldesmon 1 (CALD1))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Caldesmon est non-conjugé

Application: Western Blotting (WB)

Clone: RCALD1-820

Détail du produit anti-Caldesmon anticorps

Fonction: Recombinant Caldesmon, HMW (h-Caldesmon) (Smooth Muscle Marker) Antibody

Specificité: Recognizes a protein of 150 kDa, which is identified as the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120 150 kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70 80 kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This MAb recognizes only the 150 kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.

Réactivité croisée (Details): Human. Does not react with rat. Others not known.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Recombinant full-length human CALD1 protein

Isotype: IgG1, kappa

Information d'application

Indications d'application:

Known_Application: Western Blot (1-2 μg/mL), ,Immunohistology (Formalin-fixed) (0.25-0.5 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Ovary, Uterus, Blood vessels in all tissues, smooth muscle or leiomyosarcoma

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur Caldesmon

Antigène: Caldesmon (CALD1) (Caldesmon 1 (CALD1))

Autre désignation: CALD1

Sujet:

CAD, CALD1, Caldesmon 1 Isoform 1, Caldesmon 1 Isoform 2, Caldesmon 1 Isoform 3, Caldesmon 1 Isoform 4, Caldesmon 1 Isoform 5, CDM, HCAD, LCAD, NAG22

Blood vessels in all tissues, ovary, smooth muscle or leiomyosarcoma, Uterus

Poids moléculaire: 120-150kDa

ID gène: 800, 490203

UniProt: Q05682

Pathways: Myometrial Relaxation and Contraction