Recombinant Cyclin E1 anticorps (AA 10-176)

N° du produit ABIN7127123

Cet anticorps anti-Cyclin E1 est un anticorps Souris Monoclonal détectant Cyclin E1 dans WB et IHC (f). Adapté pour Humain.

Aperçu rapide pour Recombinant Cyclin E1 anticorps (AA 10-176) (ABIN7127123)

Antigène: Cyclin E1 (CCNE1)

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Cyclin E1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Détail du produit anti-Cyclin E1 anticorps

Épitope: AA 10-176

Specificité: Cyclin E belongs to the highly conserved cyclin family, whose members exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclins function as regulators of CDK kinases. Cyclin E forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. Cyclin E accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Cyclin E overexpression has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis.

Réactivité croisée (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant human Cyclin E (CCNE1) protein fragment (around aa 10-176) (exact sequence is proprietary)

Isotype: IgG1

Information d'application

Indications d'application:

Known_Application: Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Jurkat, HCT116 or HeLa cells. Human breast or colon carcinoma.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur Cyclin E1

Antigène: Cyclin E1 (CCNE1)

Autre désignation: CCNE1

Sujet: CCNE1, Cyclin E1, G1/S-specific cyclin-E1,Cyclin E (G1/S-Phase Cyclin)
Cellular localisation: Nuclear

Poids moléculaire: 53kDa

ID gène: 898, 244723

Pathways: Cycle Cellulaire, Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Mitotic G1-G1/S Phases