CD33 anticorps (AA 51-224)

N° du produit ABIN7127193

Cet anticorps Souris Monoclonal détecte spécifiquement CD33 dans IHC (f). Il présente une réactivité envers Humain.

Aperçu rapide pour CD33 anticorps (AA 51-224) (ABIN7127193)

Antigène: CD33

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD33 est non-conjugé

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Détail du produit anti-CD33 anticorps

Épitope: AA 51-224

Specificité: Recognizes a 67 kDa glycoprotein, which is identified as CD33. It is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies, these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia. CD33 is expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells. It is absent on platelets, lymphocytes, erythrocytes and hematopoietic stem cells.

Réactivité croisée (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant fragment (around aa51-224) of human CD33 protein (exact sequence is proprietary)

Isotype: IgG2

Information d'application

Indications d'application:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human dendritic and mast cells. Human lymph nodes and tonsils.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur CD33

Antigène: CD33

Autre désignation: CD33

Sujet: Gp67, My9, Myeloid cell surface antigen CD33, p67, Sialic acid-binding Ig-like lectin 3 (SIGLEC3),CD33 / SIGLEC3 (Myeloid Cell Surface Antigen)
Cellular localisation: Cell surface

Poids moléculaire: 67kDa

ID gène: 945, 83731

UniProt: P20138