Recombinant CD74 anticorps

N° du produit ABIN7127225

Cet anticorps anti-CD74 Monoclonal Souris (Clone rCLIP-813) (ABIN7127225) détecte spécifiquement CD74 dans WB et IHC. L’anticorps est réactif avec des échantillons de Humain, Souris et Babouin.

Aperçu rapide pour Recombinant CD74 anticorps (ABIN7127225)

Antigène: CD74 (HLA-DR-gamma (CD74))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain, Souris, Babouin

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD74 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: RCLIP-813

Détail du produit anti-CD74 anticorps

Fonction: Recombinant CD74 (B-Cell Marker) Antibody

Specificité: It recognizes a protein of ~35 kDa, identified as CD74 (Workshop IV). CD74 is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages, and monocytes, and many epithelial cells. Anti-CD74 stains predominantly germinal center lymphocytes and B-cell lymphomas, but rarely T-cell lymphomas. Anti-CD74 has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+).

Réactivité croisée (Details): Human. Baboon. Mouse. Does not react with rat.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Recombinant full-length human CD74 protein

Isotype: IgG1, kappa

Information d'application

Indications d'application:

Known_Application: Western Blot (2-4 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: 293T, Daudi or Raji cells. Tonsil or lymph node.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur CD74

Antigène: CD74 (HLA-DR-gamma (CD74))

Autre désignation: CD74

Sujet:

DHLAG, Gamma chain of class II antigens, HLA class II histocompatibility antigen gamma chain, HLA-DR antigens-associated invariant chain, HLADR-gamma (HLADG), Ia antigen-associated invariant chain, la-gamma, Major histocompatibility complex class II invariant chain, MHC HLA-DR gamma chain

293T, Daudi or Raji cells. Tonsil or lymph node. Human Spleen.

Poids moléculaire: 33-41kDa

ID gène: 972, 436568

UniProt: P044233

Pathways: Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Negative Regulation of intrinsic apoptotic Signaling, Cancer Immune Checkpoints