CDK1 anticorps

N° du produit ABIN7127233

L’anticorps Souris Monoclonal anti-CDK1 a été validé pour WB et IHC (f). Il convient pour détecter CDK1 dans des échantillons de Humain.

Aperçu rapide pour CDK1 anticorps (ABIN7127233)

Antigène: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CDK1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Détail du produit anti-CDK1 anticorps

Specificité: Recognizes a 34 kDa protein, which is identified as cyclin dependent kinase 1 (cdk1) or p34cdc2 protein kinase. cdk1 / p34cdc2 plays a crucial role during cell division and is most active during mitosis. It is predominantly localized in the nucleus. It is a serine/threonine kinase, which is activated by cyclin, presumably by de-phosphorylation of tyrosine residues. Activated cdk1 / p34cdc2 performs specific functions during mitosis, including nuclear envelope breakdown and chromosome condensation.

Réactivité croisée (Details): Human. Monkey. Cow. Mink. Does not react with Mouse, Rat, Xenopus, drosophila, S, pombe or S. cerevisiae.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant full-length human p34cdc2 protein

Isotype: IgG2a

Information d'application

Indications d'application:

Known_Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (2-4 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or MCF-7 cells. Tonsil.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur CDK1

Antigène: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Autre désignation: CDC2

Sujet: Cdc2, CDC28A, CDK1, CDKN1, CELL CYCLE CONTROLLER CDC2, Cell division control protein 2 homolog, Cell division cycle 2 G1 to S and G2 to M, Cell division protein kinase 1, Cyclin-dependent kinase 1, p34 Protein Kinase, P34CDC2,Cdk1 / p34cdc2
Cellular localisation: Nuclear and Cytoplasmic

Poids moléculaire: 34kDa

ID gène: 983, 334562

UniProt: P06493

Pathways: Cycle Cellulaire, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Toll-Like Receptors Cascades, Synthesis of DNA