Recombinant PRKDC anticorps (pSer2056)

N° du produit ABIN7127732

Cet anticorps Lapin Monoclonal détecte spécifiquement PRKDC dans ELISA et IF. Il présente une réactivité envers Humain.

Aperçu rapide pour Recombinant PRKDC anticorps (pSer2056) (ABIN7127732)

Antigène: PRKDC (Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp PRKDC est non-conjugé

Application: ELISA, Immunofluorescence (IF)

Clone: 4A4

Détail du produit anti-PRKDC anticorps

Épitope: pSer2056

Fonction: Phospho-PRKDC (S2056) Recombinant Monoclonal Antibody

Purification: Affinity-chromatography

Immunogène: A synthesized peptide derived from human Phospho-PRKDC (S2056)

Isotype: IgG

Information d'application

Indications d'application: IF:1:20-1:200

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C,-80 °C

Stockage commentaire: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Détails sur PRKDC

Antigène: PRKDC (Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC))

Autre désignation: PRKDC

Sujet:

Background: Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. Phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2. Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA. Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D. Contributes to the determination of the circadian period length by antagonizing phosphorylation of CRY1 'Ser-588' and increasing CRY1 protein stability, most likely through an indirect mechanism. Interacts with CRY1 and CRY2, negatively regulates CRY1 phosphorylation. Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway.

Aliases: DNA-dependent protein kinase catalytic subunit, DNA-PK catalytic subunit, DNA-PKcs, DNPK1, p460, PRKDC, HYRC, HYRC1

UniProt: P78527

Pathways: Réparation de l'ADN, Production of Molecular Mediator of Immune Response