PKC theta anticorps (pThr538)

N° du produit ABIN711746

Il existe 1 publication pour ce produit. L’anticorps anti-PKC theta Polyclonal Lapin est utilisé pour la détection de PKC theta dans des échantillons de Humain. Il a été validé pour WB, IHC (p), IF (cc), IF (p) et IHC (fro).

Aperçu rapide pour PKC theta anticorps (pThr538) (ABIN711746)

Antigène: PKC theta (PRKCQ) (Protein Kinase C, theta (PRKCQ))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PKC theta est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-PKC theta anticorps

Épitope: pThr538

Fonction: phospho-PRKCQ (Thr538) Polyclonal Antibody

Réactivité croisée: Humain, Souris, Rat

Homologie: Dog

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic phosphopeptide derived from human PRKCQ around the phosphorylation site of Thr538

Isotype: IgG

Information d'application

Indications d'application: WB(1:300-5000),

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Date de péremption: 12 months

Références pour anticorps anti-PKC theta (ABIN711746)

van Rees, Lago, Cox, Tomasik, Rustogi, Weigelt, Ozcan, Cooper, Drexhage, Leweke, Bahn: "Evidence of microglial activation following exposure to serum from first-onset drug-naïve schizophrenia patients." dans: Brain, behavior, and immunity, Vol. 67, pp. 364-373, (2018) (PubMed).

Détails sur PKC theta

Antigène: PKC theta (PRKCQ) (Protein Kinase C, theta (PRKCQ))

Autre désignation: PRKCQ

Sujet:

Synonyms: PRKCT, nPKC-theta, Protein kinase C theta type, PRKCQ

Background: Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that mediates non-redundant functions in T-cell receptor (TCR) signaling, including T-cells activation, proliferation, differentiation and survival, by mediating activation of multiple transcription factors such as NF-kappa-B, JUN, NFATC1 and NFATC2. In TCR-CD3/CD28-co-stimulated T-cells, is required for the activation of NF-kappa-B and JUN, which in turn are essential for IL2 production, and participates to the calcium-dependent NFATC1 and NFATC2 transactivation. Mediates the activation of the canonical NF-kappa-B pathway (NFKB1) by direct phosphorylation of CARD11 on several serine residues, inducing CARD11 association with lipid rafts and recruitment of the BCL10-MALT1 complex, which then activates IKK complex, resulting in nuclear translocation and activation of NFKB1. May also play an indirect role in activation of the non-canonical NF-kappa-B (NFKB2) pathway. In the signaling pathway leading to JUN activation, acts by phosphorylating the mediator STK39/SPAK and may not act through MAP kinases signaling. Plays a critical role in TCR/CD28-induced NFATC1 and NFATC2 transactivation by participating in the regulation of reduced inositol 1,4,5-trisphosphate generation and intracellular calcium mobilization. After costimulation of T-cells through CD28 can phosphorylate CBLB and is required for the ubiquitination and subsequent degradation of CBLB, which is a prerequisite for the activation of TCR. During T-cells differentiation, plays an important role in the development of T-helper 2 (Th2) cells following immune and inflammatory responses, and, in the development of inflammatory autoimmune diseases, is necessary for the activation of IL17-producing Th17 cells. May play a minor role in Th1 response.

ID gène: 5588

UniProt: Q04759

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Thromboxane A2 Receptor Signaling