p300 anticorps (N-Term)

N° du produit ABIN7184493

L’anticorps Lapin Polyclonal anti-p300 a été validé pour WB, IHC et ELISA. Il convient pour détecter p300 dans des échantillons de Humain.

Aperçu rapide pour p300 anticorps (N-Term) (ABIN7184493)

Antigène: p300 (EP300) (E1A Binding Protein P300 (EP300))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp p300 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Détail du produit anti-p300 anticorps

Épitope: N-Term

Fonction: EP300 Antibody

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogène: Synthesized peptide derived from N-terminal of Human p300.

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C,-80 °C

Stockage commentaire: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Détails sur p300

Antigène: p300 (EP300) (E1A Binding Protein P300 (EP300))

Autre désignation: EP300

Sujet:

Background: Functions as histone acetyltransferase and regulates transcription via chromatin remodeling. Acetylates all four core histones in nucleosomes. Histone acetylation gives an epigenetic tag for transcriptional activation. Mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. Mediates acetylation of histone H3 at 'Lys-122' (H3K122ac), a modification that localizes at the surface of the histone octamer and stimulates transcription, possibly by promoting nucleosome instability. Mediates acetylation of histone H3 at 'Lys-27' (H3K27ac). Also functions as acetyltransferase for nonhistone targets. Acetylates 'Lys-131' of ALX1 and acts as its coactivator in the presence of CREBBP. Acetylates SIRT2 and is proposed to indirectly increase the transcriptional activity of TP53 through acetylation and subsequent attenuation of SIRT2 deacetylase function. Acetylates HDAC1 leading to its inactivation and modulation of transcription. Acts as a TFAP2A-mediated transcriptional coactivator in presence of CITED2. Plays a role as a coactivator of NEUROD1-dependent transcription of the secretin and p21 genes and controls terminal differentiation of cells in the intestinal epithelium. Promotes cardiac myocyte enlargement. Can also mediate transcriptional repression. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes. Acetylates FOXO1 and enhances its transcriptional activity. Acetylates BCL6 wich disrupts its ability to recruit histone deacetylases and hinders its transcriptional repressor activity. Participates in CLOCK or NPAS2-regulated rhythmic gene transcription, exhibits a circadian association with CLOCK or NPAS2, correlating with increase in PER1/2 mRNA and histone H3 acetylation on the PER1/2 promoter.

Wuchao Yuan, J. Biol. Chem., Apr 1996, 271: 9009.
E. Tory Manning, Mol. Cell. Biol., Jun 2001, 21: 3876 - 3887.
Jiwen Li, Mol. Cell. Biol., Mar 2000, 20: 2031 - 2042.
Gopalswamy Jayaraman, J. Biol. Chem., Jun 1999, 274: 17342.

Aliases: E1A associated protein p300 antibody, E1A binding protein p300 antibody, E1A-associated protein p300 antibody, EP300 antibody, EP300: E1A binding protein p300 antibody, EP300_HUMAN antibody, Histone acetyltransferase p300 antibody, KAT3B antibody, p300 HAT antibody, RSTS2 antibody

UniProt: Q09472

Pathways: Signalisation p53, Signalisation Notch, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Regulation of Cell Size