Cyclin D2 anticorps (pThr280)

N° du produit ABIN7225726

L’anticorps anti-Cyclin D2 Polyclonal Lapin est utilisé pour la détection de Cyclin D2 dans des échantillons de Humain, Souris et Rat. Il a été validé pour WB, ELISA et IHC (p).

Aperçu rapide pour Cyclin D2 anticorps (pThr280) (ABIN7225726)

Antigène: Cyclin D2 (CCND2)

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Cyclin D2 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-Cyclin D2 anticorps

Épitope: pThr280

Fonction: Cyclin D2 (phospho Thr280) Polyclonal Antibody

Specificité: Phospho-Cyclin D2 (T280) Polyclonal Antibody detects endogenous levels of Cyclin D2 protein only when phosphorylated at T280.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogène: Synthesized peptide derived from human Cyclin D2 Phospho-Thr280

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.

Commentaires:

Primary Antibody

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Détails sur Cyclin D2

Antigène: Cyclin D2 (CCND2)

Autre désignation: Cyclin D2

Sujet: Rabbit Anti-Cyclin D2 (phospho Thr280) Polyclonal Antibody,CCND2, G1/S-specific cyclin-D2,The protein encoded by CCND2 (cyclin D2) belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK4 or CDK6 and functions as a regulatory subunit of the complex, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. Knockout studies of the homologous gene in mouse suggest the essential roles of this gene in ovarian granulosa and germ cell proliferation. High level expression of CCND2 was observed in ovarian and testicular tumors. Mutations in CCND2 are associated with megalencephaly-polymicrogyria-polydactyly-hydrocephalus syndrome 3 (MPPH3).,cyclin-D2

Poids moléculaire: observerd band 40kDa

ID gène: 894

UniProt: P30279

Pathways: Cycle Cellulaire, Mitotic G1-G1/S Phases