EIF4E anticorps (pSer209)

N° du produit ABIN7212948

L’anticorps anti-EIF4E Polyclonal Lapin est utilisé pour la détection de EIF4E dans des échantillons de Humain, Rat et Souris. Il a été validé pour WB, IHC, ELISA et IF.

Aperçu rapide pour EIF4E anticorps (pSer209) (ABIN7212948)

Antigène: EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp EIF4E est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Détail du produit anti-EIF4E anticorps

Épitope: pSer209

Fonction: Rabbit Anti-eIF4E (phospho Ser209) Polyclonal Antibody

Specificité: Phospho-eIF4E (S209) Polyclonal Antibody detects endogenous levels of eIF4E protein only when phosphorylated at S209.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogène: Synthesized peptide derived from human eIF4E Phospho-Ser209

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:20000,IF 1:50-200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Date de péremption: 12 months

Détails sur EIF4E

Antigène: EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Autre désignation: eIF4E

Sujet: EIF4E, EIF4EL1, EIF4F, Eukaryotic translation initiation factor 4E, eIF-4E, eIF4E, eIF-4F 25 kDa subunit, mRNA cap-binding proteinEukaryotic translation initiation factor 4E encoded by EIF4E is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of messenger RNAs.Eukaryotic translation initiation factor 4E aids in translation initiation by recruiting ribosomes to the 5'-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. EIF4E acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. Several pseudogenes of EIF4E are found on other chromosomes. Alternative splicing results in multiple transcript variants.

Poids moléculaire: 25kD

ID gène: 1977

UniProt: P06730

Pathways: BCR Signaling