RIPK2 anticorps (pTyr474)

N° du produit ABIN7232880

Cet anticorps anti-RIPK2 est un anticorps Lapin Polyclonal détectant RIPK2 dans WB et IHC. Adapté pour Humain.

Aperçu rapide pour RIPK2 anticorps (pTyr474) (ABIN7232880)

Antigène: RIPK2 (Receptor-Interacting Serine-threonine Kinase 2 (RIPK2))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RIPK2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-RIPK2 anticorps

Épitope: pTyr474

Fonction: Anti-RIPK2 Rabbit Polyclonal Antibody

Specificité: Human and mouse RIPK2.

Réactivité croisée: Humain, Souris

Purification: Whole Antiserum

Immunogène: Phosphopeptide corresponding to the pY474 site of human RIPK2.

Information d'application

Indications d'application: Immunoblotting: use at a dilution of 1:500. Detection of RIPK2 in MDP-stimulated HEK293 cells (+) vs. non-stimulated HEK293 cells (-)
These are recommended dilutions.
Endusers should determine optimal dilutions for their applications.
Immunohistochemistry: use at a dilution of 1:100. Detection of active RIPK2 in human (top) and mouse (bottom) tissues. Non-IBD refers to colon sections from non-inflammatory bowel disease (IBD) patients, IBD refers to colon sections from ulcerative colitis (UC) patients. Mouse strain used: B6.129P2-I110tm1Cgn/J, a common model for IBD that presents with spontaneous colitis that is elevated by DSS (dextran sulfate sodium) exposure.

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: Dilute in PBS or medium that is identical to that used in the assay system.

Concentration: Lot specific

Buffer: Whole antiserum

Stock: -20 °C

Stockage commentaire: Store at -20°C in appropriate aliquots to avoid multiple freeze-thaw cycles.

Détails sur RIPK2

Antigène: RIPK2 (Receptor-Interacting Serine-threonine Kinase 2 (RIPK2))

Autre désignation: RIPK2

Sujet: Receptor-interacting serine/threonine-protein kinase 2,Serine/threonine/tyrosine kinase that plays an essential role in modulation of innate and adaptive immune responses. Upon stimulation by bacterial peptidoglycans, NOD1 and NOD2 are activated, oligomerize and recruit RIPK2 through CARD-CARD domains. Contributes to the tyrosine phosphorylation of the guanine exchange factor ARHGEF2 through Src tyrosine kinase leading to NF-kappaB activation by NOD2. Once recruited, RIPK2 autophosphorylates and undergoes 'Lys-63'-linked polyubiquitination by E3 ubiquitin ligases XIAP, BIRC2 and BIRC3. The polyubiquitinated protein mediates the recruitment of MAP3K7/TAK1 to IKBKG/NEMO and induces 'Lys-63'-linked polyubiquitination of IKBKG/NEMO and subsequent activation of IKBKB/IKKB. In turn, NF-kappa-B is released from NF-kappa-B inhibitors and translocates into the nucleus where it activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Plays also a role during engagement of the T-cell receptor (TCR) in promoting BCL10 phosphorylation and subsequent NF-kappa-B activation. Plays a role in the inactivation of RHOA in response to NGFR signaling (PubMed:26646181). {PubMed:14638696, PubMed:17054981, PubMed:18079694, PubMed:21123652, PubMed:21887730, PubMed:26646181}.,Cytoplasm

ID gène: 603455

UniProt: O43353

Pathways: TCR Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Toll-Like Receptors Cascades