IGF1 anticorps (Secreted)

N° du produit ABIN7237701

Cet anticorps Lapin Polyclonal détecte spécifiquement IGF1 dans WB et IHC. Il présente une réactivité envers Rat.

Aperçu rapide pour IGF1 anticorps (Secreted) (ABIN7237701)

Antigène: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp IGF1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-IGF1 anticorps

Épitope: AA 74-85, Secreted

Fonction: A Rabbit Polyclonal antibody to IGF1

Specificité: Secreted

Réactivité croisée: Humain, Souris, Rat

Réactivité croisée (Details): The antibody is specific for IGF1, it does not crossreact with IGF2.

Homologie: Mouse - identical, Human - 11 out of 12 amino acid residues identical

Attributs du produit: Secreted

Purification: Affinity purified on immobilized antigen.

Immunogène:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)NKPTGYGSSIRR, corresponding to amino acid residues 74 - 85 of rat IGF1

Isotype: IgG

Information d'application

Indications d'application:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:200

Commentaires:

Negative Control: (ABIN7235814)

Blocking Peptide: (ABIN7235814)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Stock: 4 °C,-20 °C

Stockage commentaire:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Détails sur IGF1

Antigène: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Autre désignation: IGF1 

Sujet: Insulin Like Growth Factor 1, Insulin-Like Growth Factor I, IGF-I, Somatomedin-C,IGF-1 originally called somatomedin C is a 70-amino acid hormone with homology to IGF-2 and proinsulin. IGF-1 acts as an endocrine, paracrine, and autocrine hormone and is known as a mediator of prenatal and postnatal growth. IGF-1 is mainly synthesized in the liver and regulated by growth hormones (GH) in peripheral tissues such as muscle, cartilage, bone, kidney, nerves, skin, lungs, and the liver itself. The synthesized IGF-1 binds to IGF-1 binding proteins (IGFBPs) with high affinity, IGFBPs regulates the interactions of IGF-1 and its receptor (IGF-1R)1. Binding of IGF-1 to his receptor results in autophosphorylation of the receptor leading to activation of the insulin receptor substrate 1 (IRS1) protein. The activated receptor contains several phosphotyrosine sites that serve as docking sites for many signaling proteins. The association of these proteins together start a complex signaling pathway including the canonical PI3-kinase and mitogen-activated protein (MAP) kinase pathways2.The IGF-1 signaling pathway is a major regulator of the aging process, from rodents to humans3. IGF-1 deficiency follows an autosomal mode of transmission and different mutations can lead to either IGF-1 deletion or low levels of circulation IGF-1. Studies demonstrate IGF-1 deficit can lead to deregulated lipid metabolism, cardiovascular disease (CVD), diabetes, and altered metabolic profile of diabetic patients4.

Alternative names: Insulin Like Growth Factor 1, Insulin-Like Growth Factor I, IGF-I, Somatomedin-C

ID gène: 24482

NCBI Accession: NM_000618

UniProt: P08025

Pathways: Signalisation RTK, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation