ARNT anticorps

N° du produit ABIN7384450

Cet anticorps anti-ARNT est un anticorps Lapin Monoclonal détectant ARNT dans WB et IHC (p). Adapté pour Humain, Souris, Rat et Hamster.

Aperçu rapide pour ARNT anticorps (ABIN7384450)

Antigène: ARNT (Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT))

Reactivité: Humain, Souris, Rat, Hamster

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp ARNT est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: R05-3B2

Détail du produit anti-ARNT anticorps

Purification: Affinity Purified

Isotype: IgG

Information d'application

Indications d'application: WB 1:500-1:1000 IHC 1:50-1:100

Restrictions: For Research Use only

Stockage

Concentration: 300 μg/mL

Buffer: 50 mM Tris-Glycine( pH 7.4), 0.15M NaCl, 40 % Glycerol, 0.01 % Sodium azide and 0.05 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Avoid freeze / thaw cycles.

Détails sur ARNT

Antigène: ARNT (Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT))

Autre désignation: HIF1 beta

Sujet: ArnT,ARNT protein,ARNT,Aryl hydrocarbon receptor nuclear translocator,bHLHe2,Class E basic helix-loop-helix protein 2,Dioxin receptor,Dioxin receptor nuclear translocator,Drnt,HIF 1 beta,HIF 1beta,HIF-1-beta,HIF1-beta,HIF1B,HIF1beta,Hypoxia Inducible Factor 1,Hypoxia inducible factor 1 beta,Hypoxia-inducible factor 1-beta,nuclear translocator,Tango,Hypoxia-inducible factor 1 (HIF1) is a heterodimeric transcription factor that plays a critical role in the cellular response to hypoxia (1). The HIF1 complex consists of two subunits, HIF-1α and HIF-1β, which are basic helix-loop-helix proteins of the PAS (Per, ARNT, Sim) family (2). HIF1 regulates the transcription of a broad range of genes that facilitate responses to the hypoxic environment, including genes regulating angiogenesis, erythropoiesis, cell cycle, metabolism and apoptosis. The widely expressed HIF-1α is typically degraded rapidly in normoxic cells by the ubiquitin/proteasomal pathway. Under normoxic conditions, HIF-1α is proline hydroxylated leading to a conformational change that promotes binding to the von Hippel Lindau protein (VLH) E3 ligase complex, ubiquitination and proteasomal degradation follows (3,4). Both hypoxic conditions and chemical hydroxylase inhibitors (such as desferrioxamine and cobalt) inhibit HIF-1α degradation and lead to its stabilization. In addition, HIF-1α can be induced in an oxygen-independent manner by various cytokines through the PI3K-AKT-mTOR pathway (5-7).HIF-1β is also known as AhR nuclear translocator (ARNT) due to its ability to partner with the aryl hydrocarbon receptor (AhR) to form a heterodimeric transcription factor complex (8). Together with AhR, HIF-1β plays an important role in xenobiotics metabolism (8).

Poids moléculaire:

Observed_MW: 87kDa

Calculated_MW: 87kDa

ID gène: 405

UniProt: P27540

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, L'effet Warburg