PUMA anticorps (C-Term)

N° du produit ABIN7464435

Cet anticorps Lapin Polyclonal détecte spécifiquement PUMA dans WB, IP et IHC (p). Il présente une réactivité envers Humain.

Aperçu rapide pour PUMA anticorps (C-Term) (ABIN7464435)

Antigène: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PUMA est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-PUMA anticorps

Épitope: C-Term

Réactivité croisée: Humain

Purification: Purified by antigen-affinity chromatography.

Immunogène: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PUMA. The exact sequence is proprietary.

Isotype: IgG

Information d'application

Indications d'application: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Commentaires:

Positive Control: HCT116 cells with 30uM cisplatin treatment for 24hr , K562 , THP-1

Validation: Orthogonal

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Agent conservateur: Thimerosal (Merthiolate)

Précaution d'utilisation: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Détails sur PUMA

Antigène: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Autre désignation: BCL2 binding component 3

Sujet: BCL2 binding component 3 , JFY-1 , JFY1 , PUMA,This gene encodes a member of the BCL-2 family of proteins. This family member belongs to the BH3-only pro-apoptotic subclass. The protein cooperates with direct activator proteins to induce mitochondrial outer membrane permeabilization and apoptosis. It can bind to anti-apoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. Because of its pro-apoptotic role, this gene is a potential drug target for cancer therapy and for tissue injury. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2011]

ID gène: 27113

UniProt: Q96PG8, Q9BXH1

Pathways: Signalisation p53, Positive Regulation of Endopeptidase Activity