MAP3K3 anticorps

N° du produit ABIN7465309

L’anticorps Lapin Polyclonal anti-MAP3K3 a été validé pour WB et IHC (p). Il convient pour détecter MAP3K3 dans des échantillons de Humain.

Aperçu rapide pour MAP3K3 anticorps (ABIN7465309)

Antigène: MAP3K3 (Mitogen-Activated Protein Kinase Kinase Kinase 3 (MAP3K3))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MAP3K3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-MAP3K3 anticorps

Réactivité croisée: Humain, Souris

Purification: Purified by antigen-affinity chromatography.

Immunogène: Recombinant protein encompassing a sequence within the center region of human MEKK3. The exact sequence is proprietary.

Isotype: IgG

Information d'application

Indications d'application: WB: 1:5000-1:20000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Commentaires:

Positive Control: 3xFlag-human MAP3K3-transfected 293T

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.71 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Agent conservateur: Thimerosal (Merthiolate)

Précaution d'utilisation: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Détails sur MAP3K3

Antigène: MAP3K3 (Mitogen-Activated Protein Kinase Kinase Kinase 3 (MAP3K3))

Autre désignation: mitogen-activated protein kinase kinase kinase 3

Sujet: Mitogen-activated protein kinase kinase kinase 3 , MAPKKK3 , MEKK3,This gene product is a 626-amino acid polypeptide that is 96.5 % identical to mouse Mekk3. Its catalytic domain is closely related to those of several other kinases, including mouse Mekk2, tobacco NPK, and yeast Ste11. Northern blot analysis revealed a 4.6-kb transcript that appears to be ubiquitously expressed. This protein directly regulates the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by activating SEK and MEK1/2 respectively, it does not regulate the p38 pathway. In cotransfection assays, it enhanced transcription from a nuclear factor kappa-B (NFKB)-dependent reporter gene, consistent with a role in the SAPK pathway. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq]

Poids moléculaire: 71 kDa

ID gène: 4215

UniProt: Q99759

Pathways: Signalisation MAPK