RAD51 anticorps (AA 1-338)

N° du produit ABIN7468272

Cet anticorps Souris Monoclonal détecte spécifiquement RAD51 dans WB, IHC, IP, IHC (p), IF, ICC et PLA. Il présente une réactivité envers Humain.

Aperçu rapide pour RAD51 anticorps (AA 1-338) (ABIN7468272)

Antigène: RAD51 (DNA Repair Protein Homolog 1 (RAD51))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp RAD51 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunocytochemistry (ICC), Proximity Ligation Assay (PLA)

Classe de qualité: KO Validated

Clone: 14B4

Détail du produit anti-RAD51 anticorps

Épitope: AA 1-338

Réactivité croisée: Poulet, Humain, Souris, Rat

Purification: Purified by antigen-affinity chromatography.

Immunogène: Full length (amino acids 1-338) Rad51 expressed in E. coli.

Isotype: IgG2b

Information d'application

Indications d'application: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Commentaires:

Positive Control: Neuro2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat-2 Validation: KO/KD, Orthogonal

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, No Preservative

Agent conservateur: Without preservative

Stock: 4 °C,-20 °C

Stockage commentaire: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Détails sur RAD51

Antigène: RAD51 (DNA Repair Protein Homolog 1 (RAD51))

Autre désignation: RAD51 recombinase

Sujet:

Synonyms: RAD51 recombinase , BRCC5 , FANCR , HRAD51 , HsRad51 , HsT16930 , MRMV2 , RAD51A , RECA

Background: The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009]

Poids moléculaire: 37 kDa

ID gène: 5888

UniProt: Q06609

Pathways: Réparation de l'ADN