Recombinant CD4 anticorps (AA 216-396)

N° du produit ABIN7477393

Cet anticorps anti-CD4 est un anticorps Lapin Monoclonal détectant CD4 dans IHC. Adapté pour Humain.

Aperçu rapide pour Recombinant CD4 anticorps (AA 216-396) (ABIN7477393)

Antigène: CD4

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp CD4 est non-conjugé

Application: Immunohistochemistry (IHC)

Clone: MSVA-004R

Détail du produit anti-CD4 anticorps

Épitope: AA 216-396

Immunogène: Recombinant fragment (around aa 216-396) of human CD4 protein (exact sequence is proprietary)

Isotype: IgG

Information d'application

Indications d'application: IHC 1:100-1:200

Commentaires:

Positive Control: Tonsil: A moderate to strong membranous CD4 immunostaining should be seen in a subset of T-lymphocytes which is predominantly located in the interfollicular area. Macrophages in the germinal centres should show a moderate CD4 positivity. Liver: Kupffer cells and endothelial cells in the liver sinusoids must show at least a moderate CD4 staining.

Negative Control: Tonsil: B-cells and epithelial cells must not show any CD4 staining. Liver: Hepatocytes must not show any CD4 staining.

Protocole: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Stockage

Format: Liquid

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Détails sur CD4

Antigène: CD4

Autre désignation: CD4

Sujet: L3T4, Leu3, Ly-4, Lymphocyte antigen CD4, p55, T cell antigen T4/LEU3, T cell differentiation antigen L3T4, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4,CD4 antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P01730

Pathways: TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events