Mesothelin anticorps (AA 273-407)

N° du produit ABIN7477521

Cet anticorps Souris Monoclonal détecte spécifiquement Mesothelin dans IHC. Il présente une réactivité envers Humain.

Aperçu rapide pour Mesothelin anticorps (AA 273-407) (ABIN7477521)

Antigène: Mesothelin (MSLN)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Mesothelin est non-conjugé

Application: Immunohistochemistry (IHC)

Clone: MSVA-235M

Détail du produit anti-Mesothelin anticorps

Épitope: AA 273-407

Immunogène: Recombinant fragment (around aa 273-407) of human Mesothelin (MSLN) protein (exact sequence is proprietary)

Isotype: IgG

Information d'application

Indications d'application: IHC 1:100-1:200

Commentaires:

Positive Control: Tonsil: A fraction of squamous epithelial cells of the tonsil crypts should show strong mesothelin immunostaining. Fallopian tube: An at least moderate mesothelin positivity should be seen at the apical membrane of epithelial cells.

Negative Control: Tonsil: Squamous epithelial cells of the tonsil surface and all lymphocytes must not show mesothelin immunostaining.

Protocole: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Stockage

Format: Liquid

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Détails sur Mesothelin

Antigène: Mesothelin (MSLN)

Autre désignation: Mesothelin

Sujet: CAK1, Megakaryocyte potentiating factor, Mesothelin, MSLN, SMR, SMRP,Mesothelin antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: Q13421

Pathways: EGFR Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process