Recombinant PD-L1 anticorps
Aperçu rapide pour Recombinant PD-L1 anticorps (ABIN7477557)
Antigène
Voir toutes PD-L1 AnticorpsType d'anticorp
Reactivité
Hôte
Clonalité
Conjugué
Application
Clone
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Immunogène
- KLH-conjugated linear peptide corresponding to human PD-L1
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Isotype
- IgG
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Indications d'application
- IHC 1:100-1:200
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Commentaires
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Positive Control: Alveolar macrophages, tonsil crypt epithelium and the placental cytotrophoblast show strong, predominantly membranous positivity. Follicle centre macrophages should show at least moderate intensity staining.
Negative Control: Appendix (negative staining of all epithelial cells)
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Protocole
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
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Restrictions
- For Research Use only
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Format
- Liquid
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Stock
- 4 °C,-20 °C,-80 °C
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Stockage commentaire
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- PD-L1 (CD274 (PD-L1))
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Autre désignation
- PD-L1
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Sujet
- B7 homolog 1, B7-H1, CD274, PD-L1, PDCD1 ligand 1, PDCD1L1, PDCD1LG1, Programmed cell death 1 ligand 1,PD-L1 antibody validated for immunohistochemistry on 76 different Normal Tissues
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UniProt
- Q9NZQ7
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Pathways
- Cancer Immune Checkpoints
Antigène
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