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E. Coli anticorps (FITC)

Cet anticorps Chèvre Polyclonal détecte spécifiquement E. Coli dans Func. Il présente une réactivité envers E. coli.
N° du produit ABIN7478177

Aperçu rapide pour E. Coli anticorps (FITC) (ABIN7478177)

Antigène

E. Coli

Reactivité

  • 49
  • 11
E. coli

Hôte

  • 21
  • 21
  • 17
  • 1
Chèvre

Clonalité

  • 34
  • 21
Polyclonal

Conjugué

  • 29
  • 10
  • 9
  • 9
  • 2
  • 1
Cet anticorp E. Coli est conjugé à/à la FITC

Application

  • 23
  • 22
  • 22
  • 18
  • 7
  • 5
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
Functional Studies (Func)
  • Specificité

    Many "O" and "K" antigenic serotypes of Escherichia coli

    Réactivité croisée (Details)

    Antiserum is unabsorbed and does crossreact with related Enterobacteriaceae

    Purification

    This product consists of purified IgG fraction of the neat antiserum covalently coupled with high purity Isomer I of fluorescein isothiocyanate. Care is taken to ensure complete removal of any free fluorescein from the final product.

    Immunogène

    Mixture of E. coli serotypes

    Isotype

    IgG
  • Indications d'application

    TITER : > 1:1,600 by indirect IFA vs serotypes O157:H7, O20, O125, O55, O111 & K12
    Applications for this product include direct FA staining of target antigens in a permissive tissue culture system. Working dilutlion must be determined by the user for his or her application but a starting range of 1:10 - 1:50 is suggested. Acetone fixation of the antigen source is recommended prior to staining. We also recommend use of COUNTERSTAIN/BLOCKING DILUENT (Cat.#) and MOUNTING MEDIUM (Cat.#) for optimum product performance. Enzyme amplification following reaction with FITC conjugate can also be accomplished utilizing enzyme-antibody conjugates specific to FITC (Cat.#s & ).

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    The product is formulated in a phosphate saline buffer (0.01M, pH 7.2) containing bovine serum albumin (10 mg/mL) as a stabilizer and 0.1 % sodium azide preservative.

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Stock

    4 °C,-20 °C

    Stockage commentaire

    Recommened short term (<6 months) storage is liquid at 2-8°C under subdued light. For longer term storage, aliquot and freeze.
  • Antigène

    E. Coli

    Classe de substances

    Bacteria
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