Recombinant PD-L1 anticorps

N° du produit ABIN7487752

Cet anticorps anti-PD-L1 est un anticorps Lapin Chimeric détectant PD-L1 dans FACS et BR. Adapté pour Humain.

Aperçu rapide pour Recombinant PD-L1 anticorps (ABIN7487752)

Antigène: PD-L1 (CD274 (PD-L1))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Chimeric

Conjugué: Cet anticorp PD-L1 est non-conjugé

Application: Flow Cytometry (FACS), Blocking Reagent (BR)

Clone: 332M7

Détail du produit anti-PD-L1 anticorps

Fonction: Anti-PD-L1 [332M7], Rabbit IgG, kappa

Specificité: This antibody strongly binds human and cynomolgus monkey PD-L1 and shows weak binding for mouse PD-L1. PD-L1 is highly expressed in many human tumors. . PD-L1 exerts an immune suppressive signal through binding to PD-1 and B7.1, and tumor expression of PD-L1 can mediate cancer immune evasion. Blocking this pathway, therefore provides a rationale for cancer immunotherapy.

Réactivité croisée: Cynomolgus, Souris

Attributs du produit:

Original Species of Ab: Human

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogène: The original parental antibody 332M1 was generated by immunizing BALB/c mice with recombinant human PD-L1. Later on the humanized version of 332M1 was generated (332M7) by grafting the CDRs of the original mouse antibody onto human framework regions.

Isotype: IgG kappa

Information d'application

Indications d'application: The binding characterization of this antibody towards human, mouse and cynomolgus monkey PD-L1 was done using flow cytometry. This antibody was shown to block the binding of hPD-1-Fc protein to hPD-L1 expressed on the surface of the HEK-293T cells at a concentration of 10 μg/mL. The mouse version of this antibody was reported to inhibit patient derived melanoma tumor in tumor-bearing mice. There was also found an increase in the gene expression of CD8 and IFN-γ in tumor samples from mice treated with mouse version of this antibody (US20180346571).

Commentaires:

This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détails sur PD-L1

Antigène: PD-L1 (CD274 (PD-L1))

Autre désignation: PD-L1

Sujet: CD274, PDL1, B7-H1, B7H1, B7 homolog 1, hPD-L1, PDCD1L1, PDCD1LG1, PDCD1 ligand 1, Programmed cell death 1 ligand 1, Programmed death ligand 1

UniProt: Q9NZQ7

Pathways: Cancer Immune Checkpoints