Reacts strongly with the carboxy terminus (between amino acids 3669 and 3685) of human dystrophin. Also crosssreacts strongly with skeletal, cardiac and smooth muscle dystrophin from normal mouse, rat, rabbit, dog, chicken and hamster. No crossreactivity with mdx mouse tissue. Crossreacts very weakly with pig dystrophin.
Attributs du produit
This product is intended for the qualitative identification by light microscopy of Dystrophin (C-terminus) by immunohistochemistry.
Purification
Tissue culture supernatant
Immunogène
Synthetic polypeptide consisting of the last 17 amino acids at the carboxy terminus of the human dystrophin sequence.
DMD
Reactivité: Humain
IHC, ELISA
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
Immunohistochemical (IHC) staining techniques allow for the visualization of antigens via the sequential application of a specific antibody to the antigen (primary antibody), a secondary antibody to the primary antibody and an enzyme complex with a chromogenic substrate with interposed washing steps. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site. The specimen may then be counterstained and coverslipped. Results are interpreted using a light microscope.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
The user is required to reconstitute the contents of the vial with the correct volume of sterile distilled water as indicated on the vial label.
Buffer
This product is a lyophilized tissue culture supernatant containing sodium azide as a preservative.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C
Stockage commentaire
Store at 2-8 °C. Do not freeze. Return to 2-8 °C immediately after use. Do not use after expiration date indicated on the vial label. Storage conditions other than those specified above must be verified by the user.