Beclin 1 anticorps (N-Term)

N° du produit ABIN7540604

N° du produit (Fournisseur): 600-401-mg4s

Cet anticorps Lapin Polyclonal détecte spécifiquement Beclin 1 dans WB, ELISA, IHC, IF et FM. Il présente une réactivité envers Humain.

Aperçu rapide pour Beclin 1 anticorps (N-Term) (ABIN7540604)

Antigène: Beclin 1 (BECN1) (Beclin 1, Autophagy Related (BECN1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Beclin 1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Détail du produit anti-Beclin 1 anticorps

Épitope: N-Term

N° du produit (Fournisseur): 600-401-mg4s

Fournisseur: Rockland

Fonction: Beclin 1 Antibody

Réactivité croisée (Details): This affinity purified antibody is directed against human Beclin-1.

Purification: The antibody was affinity purified from monospecific antiserum by immunoaffinity purification.

Stérilité: Sterile filtered

Immunogène: Anti-BECLIN1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a near N-terminal portion of human Beclin-1 conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotype: IgG

Information d'application

Indications d'application:

ELISA_Dilution: 1:10,000 - 1:50,000

Immunohistochemistry_Dilution: 1:100-1:200

IF_Microscopy_Dilution: 5 μg/mL

Western_Blot_Dilution: 1:1000

Commentaires:

Anti-BECLIN-1 Antibody has been tested in ELISA, WB, IF, and IHC.  Expect a band at ~51.8kDa in western blot using appropriate lysates.  Positive control used: U251-MG in WB,  HeLa in IF, Human spleen and colon cancer tissues in IHC.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détails sur Beclin 1

Antigène: Beclin 1 (BECN1) (Beclin 1, Autophagy Related (BECN1))

Autre désignation: BECN1

Sujet: Rabbit Anti-Beclin-1, Beclin1 Antibody, Beclin 1 Antibody, BECN1, Coiled-Coil Myosin-Like BCL2-Interacting Protein, Beclin 1 Autophagy Related, Beclin 1 (Coiled-Coil, Moesin-Like BCL2 Interacting Protein), ATG6 Autophagy Related 6 Homolog, Protein GT197, VPS30, GT197,BECLIN1 belongs to the beclin family. Beclin 1 plays a central role in autophagy, a catabolic process of degradation induced by starvation. Acts as core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. May play a role in antiviral host defense.  Protects against infection by a neurovirulent strain of Sindbis virus. Localized in the Golgi apparatus > trans-Golgi network membrane. Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells.  Anti-Beclin-1 Antibody is useful for researchers interested in Cancer Research including Cervix Carcinoma and Breast Cancer. 

ID gène: 8678

NCBI Accession: NP_001300927

UniProt: Q14457

Pathways: Autophagy