Connexin 43/GJA1 anticorps (Internal Region)

N° du produit ABIN7540610

N° du produit (Fournisseur): 600-401-mj2s

Cet anticorps Lapin Polyclonal détecte spécifiquement Connexin 43/GJA1 dans WB, IHC, ELISA, IF et FM. Il présente une réactivité envers Souris.

Aperçu rapide pour Connexin 43/GJA1 anticorps (Internal Region) (ABIN7540610)

Antigène: Connexin 43/GJA1 (GJA1) (Gap Junction Protein, alpha 1, 43kDa (GJA1))

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Connexin 43/GJA1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Fluorescence Microscopy (FM)

Détail du produit anti-Connexin 43/GJA1 anticorps

Épitope: Internal Region

N° du produit (Fournisseur): 600-401-mj2s

Fournisseur: Rockland

Fonction: Connexin 43 Antibody

Réactivité croisée (Details): This affinity purified antibody is directed against rat connexin 43.

Purification: The antibody was affinity purified from monospecific antiserum by immunoaffinity purification.

Stérilité: Sterile filtered

Immunogène: Anti-Connexin 43 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of mouse connexin 43 conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotype: IgG

Information d'application

Indications d'application:

ELISA_Dilution: 1:10,000 - 1:15,000

Immunohistochemistry_Dilution: 1:200

IF_Microscopy_Dilution: 15 μg/mL

Western_Blot_Dilution: 1:1000

Commentaires:

Anti-Connexin 43 Antibody has been tested in ELISA, WB, IF, and IHC. Expect a band at ~43Da in western blot using appropriate lysates. Positive control used: Mouse Heart in IHC, Rat Pup Brain cells, NIH-3T3, or HEK293T in IF, and HEK293T, Mouse Heart, or Rat Brain in WB.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détails sur Connexin 43/GJA1

Antigène: Connexin 43/GJA1 (GJA1) (Gap Junction Protein, alpha 1, 43kDa (GJA1))

Autre désignation: Gja1

Sujet: Rabbit Anti-Connexin 43 Antibody, Rabbit Anti-Gja1 Antibody, Gap Junction Protein Alpha 1, Gap Junction Protein, Alpha 1, 43 kDa, Gap Junction 43 KDa Heart Protein, Connexin-43, GJAL, Gap Junction Protein, Alpha 1, 43 kDa (Connexin 43), Oculodentodigital Dysplasia (Syndactyly Type III), Gap Junction Protein, Alpha-Like, Gap Junction Alpha-1 Protein, Connexin 43, AVSD3, EKVP3, HLHS1, PPKCA, CMDR, CX43, EKVP, ODDD, Cx43, HSS,Connexin 43 or GJA1 (Gap Junction Protein Alpha 1) is a gap junction protein that acts as a regulator of bladder capacity. Gap channels (gap junctions) are specialized cell-cell contacts that provide direct intracellular communication. They allow passive diffusion of molecules up to 1 kDa, including nutrients, metabolites (glucose), ions (K+, Ca2+) and second messengers (IP3, cAMP). It may play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph. Negative regulator of bladder functional capacity: acts by enhancing intercellular electrical and chemical transmission, thus sensitizing bladder muscles to cholinergic neural stimuli and causing them to contract. Connexin 43 may play a role in cell growth inhibition through the regulation of NOV expression and localization. It plays an essential role in gap junction communication in the ventricles. Anti-Connexin 43 Antibody is useful for researchers interested in EGF/EGFR Signaling Pathways or signaling receptor binding.

ID gène: 14609

NCBI Accession: NP_034418

UniProt: P23242

Pathways: Signalisation MAPK, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization