Recombinant CD4 anticorps

N° du produit ABIN7543051

Cet anticorps anti-CD4 Chimeric Lapin (Clone OX-68) (ABIN7543051) détecte spécifiquement CD4 dans FACS, WB, IP, SPR et Bind. L’anticorps est réactif avec des échantillons de Rat.

Aperçu rapide pour Recombinant CD4 anticorps (ABIN7543051)

Antigène: CD4

Type d'anticorp: Recombinant Antibody

Reactivité: Rat

Hôte: Lapin

Clonalité: Chimeric

Conjugué: Cet anticorp CD4 est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Surface Plasmon Resonance (SPR), Binding Studies (Bind)

Clone: OX-68

Détail du produit anti-CD4 anticorps

Fonction: Anti-CD4 [OX-68], Rabbit IgG, kappa

Specificité:

This antibody is reactive with rat CD4 domain 3 & 4, and is partly competitive with OX-67 & 69.

Application Notes original Clone: This antibody binds to rat CD4. Upon challenge with HIV-1, this antibody was able to significantly inhibit viral entry into HeLa cells expressing a mutant of rat CD4 that binds gp120 (SImon, 1997). Similarly, pre-binding of gp120 resulted in a 50 % reduction in binding of this antibody, indicating epitope masking. Analogous effects on viral entry and synctium formation are observed with MAbs against human CD4.

Attributs du produit: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Purification: Protein A affinity purified

Immunogène: This antibody was prepared by subcutaneously injecting BALB/c mice with a soluble form of rat CD4, consisting of domains 3 and 4. A boost of a mixture of free sCD4 and sCD4 bound to cells was administered intravenously.

Isotype: IgG, kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

OriginalSpeciesName: Mouse

OriginalFormat: IgG2a

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détails sur CD4

Antigène: CD4

Autre désignation: CD4

Sujet: Leu3/T4, T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, W3/25 antigen

UniProt: P05540

Pathways: TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events