Recombinant Mesothelin anticorps

N° du produit ABIN7543156

Cet anticorps anti-Mesothelin Chimeric Lapin (Clone K1) (ABIN7543156) détecte spécifiquement Mesothelin dans WB, ELISA, IHC, IF, SPR et RIA. L’anticorps est réactif avec des échantillons de Humain et Cynomolgus.

Aperçu rapide pour Recombinant Mesothelin anticorps (ABIN7543156)

Antigène: Mesothelin (MSLN)

Type d'anticorp: Recombinant Antibody

Reactivité: Humain, Cynomolgus

Hôte: Lapin

Clonalité: Chimeric

Conjugué: Cet anticorp Mesothelin est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Surface Plasmon Resonance (SPR), Radioimmunoassay (RIA)

Clone: K1

Détail du produit anti-Mesothelin anticorps

Fonction: Anti-Mesothelin [K1], Rabbit IgG, kappa

Specificité:

This antibody can bind the human mesothelium, expressed on many cancer cells like ovarian carcinoma.

Application Notes original Clone: This antibody was used for the study and characterization of CAK1 antigen present on ovarian cancers and normal mesothelium. The initial screening of the mesothelin reactive clones to live OVCAR-3 cells was done using immunofluorescence. Secondary screening was done using immunoperoxidase histochemistry on cryostat sections of normal human tissues and human tumors. Quantitative ELISA confirmed that K1 and OC125 antibodies bind to different epitopes on the OVCAR-3 cells (PMID: 1735605). This antibody can detect mesothelin in a western blot assay. Competition RIA was used to detect the presence of CAK1 antigen in the supernatent of protease or phosphatidylinositol-phospholipase C treated cells. An immunotoxin composed of K1 antibody and native Pseudomonas exotoxin was found to be selectively cytotoxic to cells expressing CAK1 antigen (PMID: 1727378). The affinity of K1 antibody to mesothelin was Kd= 8 nM and determined using surface plasmon resonance (BIAcore assay) (PMID: 9178820).

Réactivité croisée: Cynomolgus

Attributs du produit: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Purification: Protein A affinity purified

Immunogène: The original antibody was generated by immunization of mice with periodate-treated human ovarian cancinoma (OVCAR-3) cells. These mice had previously been made tolerant to normal human kidney membranes.

Isotype: IgG, kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

OriginalSpeciesName: Mouse

OriginalFormat: IgG1

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détails sur Mesothelin

Antigène: Mesothelin (MSLN)

Autre désignation: Mesothelin

Sujet: MSLN, CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor

UniProt: Q13421

Pathways: EGFR Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process