Recombinant CD19 anticorps

N° du produit ABIN7543176

L’anticorps anti-CD19 Monoclonal Souris est utilisé pour la détection de CD19 dans des échantillons de Souris et Chien. Il a été validé pour FACS, IF et ELISA.

Aperçu rapide pour Recombinant CD19 anticorps (ABIN7543176)

Antigène: CD19 (CD19 Molecule (CD19))

Type d'anticorp: Recombinant Antibody

Reactivité: Souris, Chien

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD19 est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF), ELISA

Clone: MB19-1

Détail du produit anti-CD19 anticorps

Fonction: Anti-CD19 [MB19-1], Mouse IgG1, kappa

Specificité:

This antibody recognizes murine MB19-1 (CD19), a transmembrane protein present in B lymphocytes that regulates its activation. It is required for proper differentiation of B-1 cells.

Application Notes original Clone: This clone can be used to detect and analyze the presence of the CD19 transmembrane protein. The specificity towards the protein has been confirmed using ELISA and immunofluorescence (Sato et al., 1996, PMID: 8906812). Additionally, the MB19-1 antibody has been used in several flow cytometry assays. One group used this antibody to determine the differences in CD19 distribution between cells of young and old mice (Yamamoto et al., 2018, PMID: 29625072). Another group used it to differentiate eosinophils from other leukocytes (Vanella et al., 2017, PMID: 27043413). A final group used flow cytometry to determine the fraction of B cells cells in a sample with this antibody (Bedaret et al., 2009, PMID: 19907079).

Réactivité croisée: Chien

Attributs du produit: This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgA format, for improved compatibility with existing reagents, assays and techniques.

Purification: Protein A affinity purified

Immunogène: This antibody was raised by immunising CD19-deficient mice with a cell line of mCD19+ mouse pre-B cells.

Isotype: IgG1, kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

OriginalSpeciesName: Mouse

OriginalFormat: IgA

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détails sur CD19

Antigène: CD19 (CD19 Molecule (CD19))

Autre désignation: CD19

Sujet: B-lymphocyte antigen CD19, Differentiation antigen CD19, B-lymphocyte surface antigen B4, B4, CD19 Molecule, CD19 antigen

UniProt: P25918

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway