PCNA anticorps (AA 200-250)

N° du produit ABIN723006

Cet anticorps anti-PCNA est un anticorps Souris Monoclonal détectant PCNA dans WB, IHC (p) et IF (p). Adapté pour Humain, Souris, Rat et Lapin. Ce Primary Antibody a été cité dans 4+ publications.

Aperçu rapide pour PCNA anticorps (AA 200-250) (ABIN723006)

Antigène: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reactivité: Humain, Souris, Rat, Lapin

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PCNA est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Clone: 1C11

Détail du produit anti-PCNA anticorps

Épitope: AA 200-250

Réactivité croisée: Humain, Souris, Lapin, Rat

Purification: Purified by Protein G.

Immunogène: KLH conjugated synthetic peptide derived from human PCNA

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
IHC-P 1:200-400
IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Date de péremption: 12 months

Références pour anticorps anti-PCNA (ABIN723006)

Jiang, Zhang, Ding, Duan, Lu: "Inflammation and cancer: inhibiting the progression of residual hepatic VX2 carcinoma by anti-inflammatory drug after incomplete radiofrequency ablation." dans: International journal of clinical and experimental pathology, Vol. 8, Issue 11, pp. 13945-56, (2016) (PubMed).

Zhang, Wu, Cao, Zhong, Zhou: "Generation of glucagon-like peptide-2-expressing Saccharomyces cerevisiae and its improvement of the intestinal health of weaned rats." dans: Microbial biotechnology, Vol. 9, Issue 6, pp. 846-857, (2016) (PubMed).

Hao, Zhang, Cheng, Li, Sun, Zhang, Guo, Li: "Imidapril inhibits right ventricular remodeling induced by low ambient temperature in broiler chickens." dans: Poultry science, Vol. 92, Issue 6, pp. 1492-7, (2013) (PubMed).

Chen, Shi, Shu, Xie, Yang, Zhang, Ruan, Lin, Lin, Shen, Zhang, Feng, Xie: "The effect of porcine ADM to improve the burn wound healing." dans: International journal of clinical and experimental pathology, Vol. 6, Issue 11, pp. 2280-91, (2013) (PubMed).

Détails sur PCNA

Antigène: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Autre désignation: PCNA

Sujet:

Synonyms: ATLD2, Proliferating cell nuclear antigen, PCNA, Cyclin

Background: Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.

ID gène: 5111

UniProt: P12004

Pathways: Telomere Maintenance, Réparation de l'ADN, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy