PIAS1 anticorps (Internal Region)

N° du produit ABIN7565807

L’anticorps Lapin Polyclonal anti-PIAS1 a été validé pour WB, ELISA, DB et FM. Il convient pour détecter PIAS1 dans des échantillons de Humain.

Aperçu rapide pour PIAS1 anticorps (Internal Region) (ABIN7565807)

Antigène: PIAS1 (Protein Inhibitor of Activated STAT, 1 (PIAS1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PIAS1 est non-conjugé

Application: Western Blotting (WB), ELISA, Dot Blot (DB), Fluorescence Microscopy (FM)

Détail du produit anti-PIAS1 anticorps

Épitope: Internal Region

Fonction: PIAS1 Antibody

Réactivité croisée (Details): A BLAST analysis was used to suggest reactivity to mouse 92.

Attributs du produit: Synonyms: Rabbit anti-PIAS1 Antibody, Protein Inhibitor Of Activated STAT 1, GBP, ZMIZ3, DDXBP1, DEAD/H (Asp-Glu-Ala-Asp/His) Box Binding Protein 1, GU/RH-II, E3 SUMO-protein ligase PIAS1, Gu-binding protein, GBP, AR Interacting Protein

Purification: Anti-PIAS1 Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Anti-PIAS1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide from the internal region of human PIAS1.

Immunogen Type: Conjugated Peptide

Information d'application

Indications d'application: Application Note: Anti-PIAS1 Antibody has been tested for use in ELISA, Western Blotting, and Dot Blotting. HEK293 WCL p/n (W09-000-365) and K-562 WCL p/n (W09-001-GJ7) were used as positive control lysates in western blot. Expect a band at approximately 72 kDa in Western Blots of specific cell lysates and tissues. Specific conditions for reactivity should be optimized by the end user. While not tested, it is likely Anti-PIAS1 antibody will work for immunofluorescence, IHC, and CHIP assays. Western Blot Dilution: 1:1000 ELISA Dilution: 5 μg/mL IF Microscopy Dilution: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur PIAS1

Antigène: PIAS1 (Protein Inhibitor of Activated STAT, 1 (PIAS1))

Autre désignation: PIAS1

Sujet: Background: The PIAS proteins (protein inhibitor of activated STAT) play a crucial role as transcriptional coregulators in various cellular pathways, including the STAT, p53 and the steroid hormone signaling pathway. The PIAS protein family includes at least five evolutionarily conserved genes, including PIAS1. The major function of the PIAS proteins is the control of gene transcription and can also act as small ubiquitin-like-modifier (SUMO) E3 ligases. PIAS1 binds specifically to STAT1, inhibiting STAT1-mediated gene activation and also binds to the Gu/RNA helicase II enzyme, leading to the proteolytic cleavage of Gu/RH-II. PIAS1 is a potent co-activator for CP2c-mediated alpha-globin expression in erythroid cells. Anti-PIAS1 Antibody is useful for researchers interested in epigenetics and cancer research.

ID gène: 8554

NCBI Accession: NP_057250

UniProt: O75925

Pathways: Signalistation JAK/STAT, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Muscle Cell Differentiation, Hepatitis C