TET3 anticorps (N-Term)

N° du produit ABIN7565849

L’anticorps Lapin Polyclonal anti-TET3 a été validé pour WB et ELISA. Il convient pour détecter TET3 dans des échantillons de Humain.

Aperçu rapide pour TET3 anticorps (N-Term) (ABIN7565849)

Antigène: TET3 (Tet Methylcytosine Dioxygenase 3 (TET3))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TET3 est non-conjugé

Application: Western Blotting (WB), ELISA

Détail du produit anti-TET3 anticorps

Épitope: N-Term

Fonction: TET3 Antibody

Attributs du produit: Synonyms: rabbit anti-TET3 Antibody, TET antibodies, Anti-TET Methylcytosine Dioxygenase 3, Anti-TET3 polyclonal antibody, KIAA0401 antibody, dioxygenase antibodies

Purification: Anti-TET3 Antibody was prepared from whole rabbit antiserum by delipidation and defibrination. The antiserum was further cross-absorbed against MBP by chromatography.

Stérilité: Sterile filtered

Immunogène:

Immunogen: TET3 antibody was prepared from whole rabbit serum produced by repeated immunizations with a human TET-3 domain containing the N-terminal 160 amino acids of the protein.

Immunogen Type: Recombinant Protein

Information d'application

Indications d'application: Application Note: Tet3 antibody has been tested by Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect band at ~180 kDa. This antibody is suitable for use by ELISA. Western Blot Dilution: 1:5000 ELISA Dilution: 1:50,000 Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 70 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détails sur TET3

Antigène: TET3 (Tet Methylcytosine Dioxygenase 3 (TET3))

Autre désignation: TET3

Sujet: Background: Anti-Tet3 is expressed in colon, muscle, adrenal gland and peripheral blood lymphocytes. Anti-Tet3 is involved in the epigenetic chromatin reprogramming of a zygote after its fertilization, and the demethylation occurs before the first cell division in the paternal pronucleus. Tet 3 antibody cannot bind to the maternal pronucleus chromatin due to DPPA3/PGC7 chromatin presence. Tet 3 functions as the dioxygenase which synthesizes a hydroxyl group to 5-methylcystosine (5mC) to form 5-hydroxymethylcystosine. Tet3 mediates the formation of 5-formylcystosine from 5hmC and subsequently 5fC to 5-carboxylcystosine, and consequently Tet3 plays an active role in catalyzing those conversions which have been suggested as the first step for cytosine DNA de-methylation. Tet 3 also recruits O-GlcNAc transferase OGT to aid H2B GlcNAcylation by OGT. Anti-TET3 antibodies are ideal for researchers interested in Epigenetics, Cancer, Chromatin Research and Histone research.

ID gène: 200424

NCBI Accession: NP_659430

UniProt: O43151

Pathways: L'effet Warburg