CRISPR-Cas9 (C-Term) anticorps

N° du produit ABIN7565918

L’anticorps anti-CRISPR-Cas9 Polyclonal Lapin est utilisé pour la détection de CRISPR-Cas9 dans des échantillons de Streptococcus pyogenes. Il a été validé pour WB, IF, ELISA, FM et MA.

Aperçu rapide pour CRISPR-Cas9 (C-Term) anticorps (ABIN7565918)

Antigène: CRISPR-Cas9

Reactivité: Streptococcus pyogenes

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA, Fluorescence Microscopy (FM), Multiplex Assay (MA)

Détail du produit

Épitope: C-Term

Fonction: Cas 9 Antibody

Réactivité croisée (Details): Anti-Cas9 antibody is directed against Streptococcus pyogenes Cas-9.

Attributs du produit: Synonyms: rabbit anti-Cas9 antibody, Cas-9, Cas 9, CRISPR, CRISPR-associated endonuclease Cas9/Csn1, SpCas9, SpyCas9, csn1, Cas-9 Antibody

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Cas-9 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal of Streptococcus pyogenes Cas-9 protein.

Immunogen Type: Conjugated Peptide

Information d'application

Indications d'application: Application Note: This affinity purified antibody has been tested for use in ELISA, Immunofluorescence, and Western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band ~158 kDa in size corresponding to Cas-9 protein by western blotting in the appropriate cell lysate or extract. Western Blot Dilution: 1 μg/mL ELISA Dilution: 1:25,000-1:30,000 IF Microscopy Dilution: 1 μg/mL

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détail du antigène

Antigène: CRISPR-Cas9

Autre désignation: cas9

Sujet: Background: The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR Associated (Cas) system was discovered in bacteria as a defense against foreign DNA, even being believed to act as a sort of prokaryotic immune system. The Cas9 protein is becoming a useful tool in the field of genomic editing for its ability to induce site-directed double strand breaks in DNA. It can cleave almost any sequence complementary to the guide RNA and has recently been used for modifying the genome of human embryos for the first time.

ID gène: 901176

NCBI Accession: NP_269215

UniProt: Q99ZW2