Urease anticorps (HRP)

N° du produit ABIN7565994

Cet anticorps anti-Urease est un anticorps Lapin Polyclonal détectant Urease dans WB, ELISA, IP et DB. Adapté pour Haricots-sabres.

Aperçu rapide pour Urease anticorps (HRP) (ABIN7565994)

Antigène: Urease (URE)

Reactivité: Haricots-sabres

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Urease est conjugé à/à la HRP

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Dot Blot (DB)

Détail du produit anti-Urease anticorps (HRP)

Fonction: Urease Antibody Peroxidase Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Urease [Jack Bean].

Attributs du produit: Synonyms: rabbit anti-Urease Antibody HRP Conjugation, Peroxidase Conjugated rabbit anti-Urease Antibody, Urea Amidohydrolase antibody

Purification: Urease is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Urease [Jack Bean]

Immunogen Type: Native Protein

Information d'application

Indications d'application: Application Note: Anti-Urease peroxidase conjugated antibody has been tested by dot blot and is suitable to be assayed against 1.0 μg of Urease [Jack Bean] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:500 to 1:2,000 of the reconstitution concentration is suggested for this product. Western Blot Dilution: 1:50 - 1:500 Immunoprecipitation Dilution: User Optimized ELISA Dilution: 1:500 - 1:2,000 Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détails sur Urease

Antigène: Urease (URE)

Autre désignation: Urease

Sujet: Background: Urease is an enzyme involved in nitrogen metabolism. It catalyzes the hydrolysis of urea into carbon dioxide and ammonia.

UniProt: P07374